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Recombinant Human TGF Beta-1 (TGFB1)

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Product Source Size Cat. No. Price
Recombinant Human TGF Beta-1 (TGFB1) HEK293 cells20 µg Z101555 $580.00
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Data Sheet
Print Version
Alternative Names Transforming growth factor beta-1, TGF-beta-1, Differentiation inhibiting factor, Latency-associated peptide, LAP
Description TGFB1 is the prototype member of the TGF-beta super-family that includes Inhibins, Activin A, MIS (Müllerian inhibiting substance), BMP (bone morphogenetic proteins), dpp (decapentaplegic) and Vg-1. TGFB1 is the most potent known growth inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neuronal cells, lymphoid cells and other hematopoietic cell types. TGF-beta inhibits the proliferation of T lymphocytes by down-regulating predominantly IL2 mediated proliferative signals and also inhibits the growth of natural killer cells. Recombinant human TGFB1 is a disulfide linked homodimer protein that migrates at approximately 26kDa under non-reducing conditions.
Gene Symbol TGFB1
Gene ID 7040
Accession No. P01137
Source HEK293 cells
Appearance Lyophilized Powder
Molecular Weight 26.0 kDa
Endotoxin Level <1.0 EU/μg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Bioactivity The ED50 as determined by the ability to inhibit the proliferation of mink lung epithelial cells was found to be in the range of 0.05-0.1 ng/ml
Formulation Lyophilized from 0.2 μm filtered PBS solution pH 7.4
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Product Documents
Product References
FAQs
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How are endotoxin levels measured?
1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970.

· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).

· For recombinant proteins: EU is reported per microgram of protein.

· One EU = 0.1-0.2 ng endotoxin/µg of protein.

· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?
The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.
Are your Escherichia coli sourced growth factors: 1) Human derived materials free? 2) Recombinant proteins free?
Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.