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Recombinant Human TGF Beta-3 (TGFB3)

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Product Source Size Cat. No. Price
Recombinant Human TGF Beta-3 (TGFB3) E. coli10 µg Z102515 $100.00
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Data Sheet
Print Version
Alternative Names Transforming growth factor beta-3
Description Transforming Growth Factor Beta-3 is a disulfide-linked homodimeric, non-glycosylated, protein that is one of the isoforms of the highly pleiotropic cytokines that are secreted by all cell types. TGF Beta-1, TGF Beta-2, and TGF Beta-3 use the same receptor and act as cellular switches that regulate cell growth, proliferation, differentiation, motility, extracellular matrix synthesis, and immune function regulation. Defects of this gene can cause arrhythmogenic right ventricular dysplasia 1 and rienhoff syndrome. TGFB3 first interacts with the accessory receptor betaglycan (TGF Beta-R3) and a type II serine/threonine kinase receptor (TGF Beta-R2). The receptor then activates a type I serine/threonine kinase receptor such as ALK-1 or TGF Beta-R1 (ALK-5) through phosphorylation, and the activated type I receptor activates Smad proteins to regulate transcription.
Gene Symbol TGFB3
Gene ID 7043
Accession No. P10600
Source E. coli
Appearance Liquid
Molecular Weight 13.0 kDa
Endotoxin Level <1.0 EU/μg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Gel Image Click To Enlarge
Bioactivity The ED50 is 0.01-0.04 ng/mL as determined by its ability to inhibit IL-4 dependent proliferation of HT-2 mouse cells
Formulation The filter sterilized clear protein solution contains 20% Ethanol and 0.12% Acetic acid.
Storage This cytokine can be stored in working aliquots at 2° - 8°C for two weeks. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Usage For research use only. Not for diagnostic or therapeutic use.
Product Documents
Product References
FAQs
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How are endotoxin levels measured?
1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970.

· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).

· For recombinant proteins: EU is reported per microgram of protein.

· One EU = 0.1-0.2 ng endotoxin/µg of protein.

· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?
The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.
Are your Escherichia coli sourced growth factors: 1) Human derived materials free? 2) Recombinant proteins free?
Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.