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Recombinant Human WNT1

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Product Source Size Cat. No. Price
Recombinant Human WNT1 E. coli10 µg Z102125 $190.00
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Data Sheet
Print Version
Alternative Names Proto-oncogene Int-1 homolog, Wnt-1
Recombinant Human Proto-oncogene Wnt-1 (WNT1)
Description Wnt-1 is a secreted protein that signals through the frizzled family of seven transmembrane receptors. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the up-regulation of over fifty genes. Stabilization and resulting accumulation of the intracellular signaling protein β-catenin is an important aspect of Wnt-1 signaling. While known to play in role as a developmental protein, elevated levels of Wnt proteins have also been associated with tumorigenesis.
Gene Symbol WNT1
Gene ID 7471
Accession No. P04628
Source E. coli
Appearance Lyophilized Powder
Molecular Weight 38.5 kDa
Endotoxin Level <1.0 EU/μg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Bioactivity Determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200ng/ml of human BMP-2.
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and DTT
Reconstitution Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1 - 1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (0.1% BSA) and store in working aliquots at -20 to -80⁰C
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Product Documents
Product References
FAQs
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How are endotoxin levels measured?
1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970.

· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).

· For recombinant proteins: EU is reported per microgram of protein.

· One EU = 0.1-0.2 ng endotoxin/µg of protein.

· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?
The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.
Are your Escherichia coli sourced growth factors: 1) Human derived materials free? 2) Recombinant proteins free?
Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.