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Recombinant Human sCD23 (FCER2)

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Product Source Size Cat. No. Price
Recombinant Human sCD23 (FCER2) E. coli20 µg Z101835 $70.00
1.0 mg Z101839 $1,890.00
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Data Sheet
Print Version
Alternative Names Low affinity immunoglobulin epsilon Fc receptor, BLAST-2, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, CD23, B cell differentiation antigen
Recombinant Human C-type Lectin Domain Family 4 Member J (FCER2)
Description CD23 is a calcium dependent transmembrane glycoprotein which is expressed in many cell types. The cytoplasmic region of the protein has two isoforms – the 'a' isoform that participates in IgE-regulated endocytosis, and the 'b' isoform, which is involved in IgE-regulated phagocytosis. It has been found that there are at least four soluble forms of the CD23 protein (sCD23) all of which are produced by the activity of metallo/cysteine proteases. In addition to binding IgE, sCD23 have been shown to promote the activation and differentiation of B-cells and stimulate the release of pro-inflammatory cytokines from monocytes.
Gene Symbol FCER2
Gene ID 2208
Accession No. P06734
Source E. coli
Appearance Lyophilized Powder
Molecular Weight 19.5 kDa
Endotoxin Level <1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Bioactivity Measured by its ability to bind in a functional ELISA. Immobilized rhCD23 at 2 µg/mL (100 µL/well) can bind human IgE with a linear range of 0.015­1 µg/mL.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS
Reconstitution Reconstitute at 100 μg/mL in sterile PBS
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Product Documents
Product References
FAQs
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How are endotoxin levels measured?
1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970.

· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).

· For recombinant proteins: EU is reported per microgram of protein.

· One EU = 0.1-0.2 ng endotoxin/µg of protein.

· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?
The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.
Are your Escherichia coli sourced growth factors: 1) Human derived materials free? 2) Recombinant proteins free?
Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.