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Recombinant Mouse CCL25
|Cat. No.||Product Name||Qty.||Promo/% Off||Amount|
|Alternative Names||Chemokine TECK, Small-inducible cytokine A25, Scya25, Teck, C-C motif chemokine 25
Recombinant Mouse Thymus-Expressed Chemokine (CCL25)
|Description||CCL25 is constitutively expressed in epithelium and mucosal vessels in the small bowel, where it interacts with gut-homing B and T cells expressing its receptor, CCR9.|
|Molecular Weight||14.0 kDa|
|Endotoxin Level||<1.0 EU/μg of recombinant protein as determined by the LAL method.|
|Purity||>95% as determined by SDS-PAGE|
|Bioactivity||Activity was determined by the ability to induce chemotaxis of purified human monocytes at concentrations ranging between 2-10 ng/mL.|
|Formulation||Recombinant CCL25 was was lyophilized from 0.2 μm filtered PBS, pH 7.4.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
· Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL).
· For recombinant proteins: EU is reported per microgram of protein.
· One EU = 0.1-0.2 ng endotoxin/µg of protein.
· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.