RFP Stably Expressing Immortalized Human Astrocytes, Fetal –hTERT Cell Line

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RFP Stably Expressing Immortalized Human Astrocytes, Fetal –hTERT Cell Line

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Cat.No.: T3972
Quantity: 1x106 cells/1.0ml
Price: Inquire
Qty.:
Email: order@abmGood.com
Phone: 604-247-2416 (Local), 1-866-757-2414 (Toll free)
Fax: 604-247-2414
Payment: or Purchase Order Number
More info: Addition Information Regarding Orders

Purchase Cell Line Insurance with your immortalized or stable cell line order and have peace of mind that abm will provide an additional vial should you require a replacement, no claim form needed or questions asked!

4 Month Coverage Plan
Cat. No. C192
Coverage Cells for four months from delivery of original order.
Cost $500 per cell line ordered for
Credit Options - If a replacement vial is not requested during the first four months, your insurance fees will be turned into a credit that can be applied towards any Cell Culture reagents.
- Credit valid for six months after insurance expires.

6 Month Coverage Plan
Cat. No. C193
Coverage Cells for six months from delivery of original order.
Cost $650 per cell line ordered for
Credit Options - If a replacement vial is not requested during the first six months, your insurance fees will be turned into a credit that can be applied towards any Cell Culture reagents.
- Credit valid for six months after insurance expires.
Notes:
* Please inquire prior to order placement. Not valid as Add-On after original order has been shipped.
** Insurance and/or credit does not include shipping and this is to be arranged by the customer


***Live cell shipment is available upon request for an additional $95.00 fee; please include Cat# C088 when placing your order to receive this service.***

*For for-profit organizations and corporations, please contact quotes@abmgood.com for pricing of this item.

Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at technical@abmgood.com.
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*Notes about Promo Codes: For NON-DISCOUNT promo codes (i.e. free items or shipping) please enter them during check out in the Notes/Promo Code field. To enter a DISCOUNT promo code enter it into the corresponding box in the 'Promo/% Off' column and then click 'Update' for the given product. If the code is valid for that product the discount percentage will display in place of the box and the amount will become the discounted total for that product(s). If the code is not valid it will remain in the box, please double check the expiry and spelling, and if you feel the code should still be accepted please contact us.
Catalog Number Quantity Promotion Code (Optional)
   
Data Sheet
Print Version
Organism Human
SourceOrgan Brain
BioSafety Level II
Growth Properties Adherent
Morphology Multipolar
Markers Neomycin
Propagation Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium with the final concentration: 10% fetal bovine serum (TM999), and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO2: 5%; Temperature: 37.0°C.
Procedure Overview
Freeze Thaw Recovery 1. Pre-warm complete media in a 37°C waterbath.
2. Remove the cryopreserved vial from the liquid nitrogen storage tank.
3. Thaw the cells quickly by placing the lower half of the vial into the 37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells.
4. Re-suspend the cells in the vial and transfer the cell suspension into a 15mL sterile conical tube containing 5mL complete media.
5. Centrifuge cells at 200x g for 3 minutes to pellet.
6. Aspirate out the media, leaving cell pellet undisturbed.
7. Re-suspend pellet in fresh culture medium and plate in new culture vessel.
8. Incubate cultures at 37°C, 5% CO2.

Note: Where applicable, we recommend selection drug addition after cells recovered from thawing. Directly adding selection drug while thawing could lead to stressed cells and lower viability.
Disclaimer 1. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
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