AdmiRa-hsa-let-7a-3p Virus

Cat. No.
mh1529
Unit
250 μl
Price
$245.00

Lead Time
Around 11 weeks
Cat. No. mh1529
Name AdmiRa-hsa-let-7a-3p Virus
Unit 250 μl
Description This miRNA adenovirus is part of abm’s Adenoviral Expression System and can be used directly to transiently over-express your miRNA of interest in a wide range of host cells. This adenovirus can be used to amplify more adenovirus by transducing HEK293 cells. For enhanced transduction efficiency, the use of ViralPlus (G698) at 1:100 is recommended at the time of transduction.
miRNA Name hsa-let-7a-3p
Accession Number MIMAT0004481
Species Human (H. sapiens)
Vector Map pAdeno-mir-GFP (click blue link to view)
miRNA ID MIMAT0004481
System Adenovirus
Storage Condition High titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
Disclaimer

1. abm guarantees that the correct construct is provided and GFP expression is displayed upon successful infection if the GFP construct is selected. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% infection efficiency with a positive control. The replacement will not be covered by the same guarantee.

2. Please note that due to the large number of variables applicable, any further expression analysis is not covered by the guarantee, as such analysis is dependent on the end user's experimental conditions.

3. Disclaimer for intended use: All of abm's vectors and viral particles are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its vector(s) in therapeutic/diagnostic application(s).

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. mh1529
Print & Download Datasheet
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  • Verma, M., Asakura, Y., & Asakura, A. "Inhibition of microRNA‐92a increases blood vessels and satellite cells in skeletal muscle but does not improve duchenne muscular dystrophy–related phenotype in mdx mice" Muscle & Nerve 59(5):594–602 (2019). DOI: 10.1002/mus.26433.
  • Wu., Chien-Wei., . "Downregulation of MiR-144 by Triptolide Enhanced p85α−PTEN Complex Formation Causing S Phase Arrest of Human Nasopharyngeal Carcinoma Cells" European Journal of Pharmacology 855:137-148 (2019). DOI: 10.1016/j.ejphar.2019.04.052..
  • Wu, M.-J., Chen, Y.-S., Kim, M. R., Chang, C.-C., Gampala, S., Zhang, Y., … Chang, C.-J. "Epithelial-Mesenchymal Transition Directs Stem Cell Polarity via Regulation of Mitofusin" Cell Metabolism 29(4):993–1002.e6 (2019). DOI: 10.1016/j.cmet.2018.11.004.
  • Xiang, X., Zhou, Y., Sun, H., Tan, S., Lu, Z., Huang, L., & Wang, W. "Ivabradine abrogates TNF-α-induced degradation of articular cartilage matrix" International Immunopharmacology 66:347–353 (2019). DOI: 10.1016/j.intimp.2018.11.035.
  • Zhou, Y., Lei, J., Xie, Q., Wu, L., Jin, S., Guo, B., ... & Zhang, J. "Fibrinogen-like protein 2 controls sepsis catabasis by interacting with resolvin Dp5" Science Advances 5(11):eaax0629 (2019).
  • Zhu, R., Xue, X., Shen, M., Tsai, Y., Keng, P. C., Chen, Y., … Chen, Y. "NFκB and TNFα as individual key molecules associated with the cisplatin-resistance and radioresistance of lung cancer" Experimental Cell Research 374(1):181–188 (2019). DOI: 10.1016/j.yexcr.2018.11.022.
  • Shi J, Gao X, Tian T, et al. Structural basis of Q-dependent transcription antitermination. Nat Commun. 2019;10(1):2925. Published 2019 Jul 2. doi:10.1038/s41467-019-10958-8.
  • Trachman, R., & Ferré-D'Amaré, A. (2019). Tracking RNA with light: Selection, structure, and design of fluorescence turn-on RNA aptamers. Quarterly Reviews of Biophysics, 52, E8. doi:10.1017/S0033583519000064.
  • Alexis Autour, Sunny C. Y. Jeng, Adam D. Cawte, Amir Abdolahzadeh, Angela Galli, Shanker S. S. Panchapakesan, David Rueda, Michael Ryckelynck & Peter J. Unrau "Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells" Nature Communications. 2018 Feb;23(9):656. DOI: 10.1038/s41467-018-02993-8.
  • Panchapakesan SSS, Ferguson ML, Hayden EJ, Chen X, Hoskins AA, Unrau PJ. et al. "Ribonucleoprotein purification and characterization using RNA Mango." RNA. 2017 Oct;23(10):1592-1599. DOI: 10.1261/rna.062166.117.
  • Trachman RJ 3rd, Demeshkina NA, Lau MWL, Panchapakesan SSS, Jeng SCY, Unrau PJ, Ferré-D'Amaré AR. et al. "Structural basis for high-affinity fluorophore binding and activation by RNA Mango." Nat Chem Biol. 2017 Jul;13(7):807-813. DOI: 10.1038/nchembio.2392.
  • Cawte, Adam. et al. "Live Cell Imaging of Genomic Loci using Fluorescent RNA Aptamers." Biophysical Journal. 2017 Feb, Volume 112,Issue 3,69a. DOI: 10.1016/j.bpj.2016.11.416.
  • Jeng SC, Chan HH, Booy EP, McKenna SA, Unrau PJ. et al. "Fluorophore ligand binding and complex stabilization of the RNA Mango and RNA Spinach aptamers." RNA. 2016 Dec;22(12):1884-1892. Epub 2016 Oct 24. PubMed : 27777365.
  • Ouellet J. et al. "RNA Fluorescence with Light-Up Aptamers." Front Chem. 2016 Jun 28;4:29. DOI: 10.3389/fchem.2016.00029. eCollection 2016. Review. PubMed : 27446908.
  • Panchapakesan SS, Jeng SC, Unrau PJ. et al. "RNA complex purification using high-affinity fluorescent RNA aptamer tags." Ann N Y Acad Sci. 2015 Apr;1341:149-55. DOI: 10.1111/nyas.12663. Epub 2015 Jan 13.
  • Dolgosheina EV, Jeng SC, Panchapakesan SS, Cojocaru R, Chen PS, Wilson PD, Hawkins N, Wiggins PA, Unrau PJ. et al. "RNA mango aptamer-fluorophore: a bright, high-affinity complex for RNA labeling and tracking." ACS Chem Biol. 2014 Oct 17;9(10):2412-20. DOI: 10.1021/cb500499x. Epub 2014 Aug 21.
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