AflII

CAT.NOUNITPRICE
E05010000 U (500 µl)
$135.00

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AflII
AflII

In stock

$135.00

Summary
    Specifications


    SKUE050
    Unit quantity10000 U (500 µl)
    Applications

    Restriction enzyme digestion of double-stranded DNA

    Components

    AflII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)

    Concentration20000 U/ml
    Expression SystemAn E. coli strain that carries the AflII gene from Anabaena flos-aquae (CCAP 1403/13f). The AflII enzyme recognizes and cuts the sequence C^TTAAG.
    Inactivation Protocol65°C for 20 min
    Reaction Definition1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)
    Reaction Buffer

    Buffer 1: 50%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes.

    Storage Condition

    -20°C

    Storage Buffer10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, and 50% (v/v) Glycerol
    Note

    Methylation Sensitivity
    dam methylation: Not Sensitive
    dcm methylation: Not Sensitive
    CpG Methylation: Not Sensitive

    Documents


    Supporting Protocol
    MSDS

      QC
      Other

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