AMY3 Stable HEK293 Cell Line
|T6257||1x106 cells / 1.0 ml|
|Description||Amylin receptor-3 (AMY3) is an amylin receptor that is suspected to play a role in both Abeta and hAmylin downstream signaling. Amylin receptors belong to class B GPCRs and are heterodimeric complexes formed by calcitonin receptor (CTR) association with receptor activity modifying proteins (RAMPs). Activation of amylin receptors in mammalian cells has been shown to raise cAMP and presumed to involve GS protein (Gs -guanine nucleotide-binding signal transduction protein) and stimulation of adenylate cyclase. AMY3 Stable HEK293 Cell Line allows for the study of the intracellular signal transduction pathways that are activated by Abeta or hAmylin and to further investigate whether their cytotoxic effects are mediated via this particular isoform of the amylin receptor.|
|Species||Human (H. sapiens)|
|Applications||For Research Use Only|
|Seeding Density||80,000 cells/cm2|
|Population Doubling Time||24 - 36 hours|
|Cell Type||Drug Discovery Cell Lines|
|Expression Profile||GFP expression; CTR (Calcitonin receptor); RAMP3 (receptor activity modifying protein 3)|
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
|QC||1) Immunohistochemistry; 2) RAMP gene expression; 3) Quantitative cAMP measurements; 4) Western blot; 5) Ca2 imaging; 6) MTT cell death assay|
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