Anti-EWSR1 Antibody

Y030074100 μl


Unit quantity100 μl
Raised inMouse
Species ReactivityHuman
ImmunogenEwing sarcoma breakpoint region 1 is a protein belonging to the family of RNA binding proteins which contain a single RRM domain. It also has a single Zinc Finger domain of the ZnF_RBZ subtype found in Ran binding proteins. Ran is a small G protein related to p21-Ras which regulates the import and export of proteins to the nucleus. EWSR1 appears to be a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. In particular, EWSR1 is required for cell survival in the central nervous system (1). The EWSR1 gene was identified based on its location at the chromosome 22 breakpoint of the t(11;22)(q24;q12) translocation that characterizes Ewing sarcoma and related neuroectodermal tumors. This results in the production of an aberrant fusion protein containing the N-terminus of a protein encoded by the Friend leukemia virus integration 1 (FLI1) protein and the C-terminal of the EWS protein. The chromosomal region harboring the EWS gene appears to be particularly prone to translocations, and such translocations can result in the production of several other aberrant fusion proteins, in each case with C-terminus of EWS fused with an another protein (2, see also the OMIM link). EWSR1 is ubiquitely expressed and is localized primarily in the nucleus of cell (3). It has also been found in the cytoplasm, and associated with the plasma membrane in a fashion regulated by the protein kinase Pyk2 (4). Expression of EWS in the various subcellular compartments is affected by the methylation state of its RNA-binding domain (5). Localization of EWS in different subcellular compartments reflects a dynamic distribution during cell cycle. EWS associates with and stablizes microtubules, leading to cell cycle arrest (6). EWSR1, TAF15 and FUS/TLS are closely related proteins which are believed to have evolved by gene multiplication, and all have been implication in the etiology of some forms of Amyotrophic Lateral Sclerosis (ALS, a.k.a. Lou Gehrig's Disease) (7). Monoclonal antibody MCA-5H7 was raised against full length recombinant human EWS expressed in and purified from E. coli. The HGNC name for EWS is EWSR1.
Storage ConditionThis product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage BufferPBS, pH 7.4 with 0.02% sodium azide.
Guaranteeabm guarantees that all our Anti-EWSR1 Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
PurityAffinity purified

Supporting Protocol





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