Anti-NanogRabbit Polyclonal Antibody

CAT.NOUNITPRICE
Y030044100 μl
$575.00

Specifications


DescriptionNanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells.
SKUY030044
Unit quantity100 μl
ClonePolyclonal
Raised inRabbit
ClonalityPolyclonal
IsotypeIgG
Species ReactivityHuman
ImmunogenFusion protein to NANOG with GST tag purified from E.coli
ApplicationsOptimal dilutions to be determined by researchers. Positive control found in HESC cells with expected band size of 39kDa.
Recommended Dilutions
FormulationLiquid
Concentration100ug/100ul
Storage ConditionThis product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage BufferPBS, pH 7.4 with 0.02% sodium azide.
Guaranteeabm guarantees that all our Anti-NanogRabbit Polyclonal Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
PurityAffinity purified
Documents


Supporting Protocol

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