Anti-Rabbit IgG Unlabeled Antibody (Chicken Host)

Y030100100 μg


DescriptionSince chicken IgY molecules are quite different from mammalian IgG’s, our secondary antibodies don’t cross react with primary antibodies made in rabbits, mice, or any other mammalian host tested. This makes it simple to performed double immunostaining.
Unit quantity100 μg
Raised inChicken
Species ReactivityOther
SpecificityAntibodies were tested for specificity using double-immunodiffusion in agarose. There is no detectable cross-reactivity with goat, human or mouse IgG.
ImmunogenChickens were immunized with purified, non-denatured rabbit IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-rabbit IgG’s were affinity-purified over a column conjugated with purified rabbit IgG (containing both heavy and light chains).
Applications1:1000 Western blots, 1:250 Immunohistochemistry.
Recommended Dilutions1:1000 Western blots, 1:250 Immunohistochemistry.
Storage ConditionThis product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage BufferPBS, pH 7.4 with 0.02% sodium azide.
Guaranteeabm guarantees that all our Anti-Rabbit IgG Unlabeled Antibody (Chicken Host) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
PurityAffinity purified

Supporting Protocol





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