ApaI

CAT.NOUNITPRICE
E06325000 U (500 µl)
$125.00

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ApaI
ApaI

In stock

$125.00

Summary
    Specifications


    SKUE063
    Unit quantity25000 U (500 µl)
    Applications

    Restriction enzyme digestion of double-stranded DNA

    Components

    ApaI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)

    Concentration50000 U/ml
    Expression SystemAn E. coli strain that carries the ApaI gene from Acetobacter pasteurianus sub. pasteurianus (ATCC 9432). The ApaI enzyme recognizes and cuts the sequence GGGCC^C.
    Inactivation Protocol65°C for 20 min
    Reaction Definition1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204). Incubate at 25°C. ApaI has 100% activity but a reduced half-life of 30 minutes if incubated at 37°C. ApaI is inhibited by salt concentrations higher than 50mM.
    Reaction Buffer

    Buffer 1: 25%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100%. Click here to view the buffer compatibility chart for restriction enzymes.

    Storage Condition

    -20°C

    Storage Buffer10mM Tris pH (7.5), 50mM KCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol.
    Note

    Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Blocked by overlapping CpG Methylation: Blocked by overlapping

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    Supporting Protocol
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