|E063||25000 U (500 µl)|
|Unit quantity||25000 U (500 µl)|
Restriction enzyme digestion of double-stranded DNA
ApaI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)
|Expression System||An E. coli strain that carries the ApaI gene from Acetobacter pasteurianus sub. pasteurianus (ATCC 9432). The ApaI enzyme recognizes and cuts the sequence GGGCC^C.|
|Inactivation Protocol||65°C for 20 min|
|Reaction Definition||1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204). Incubate at 25°C. ApaI has 100% activity but a reduced half-life of 30 minutes if incubated at 37°C. ApaI is inhibited by salt concentrations higher than 50mM.|
Buffer 1: 25%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100%. Click here to view the buffer compatibility chart for restriction enzymes.
|Storage Buffer||10mM Tris pH (7.5), 50mM KCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol.|
Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Blocked by overlapping CpG Methylation: Blocked by overlapping
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