|E062||2500 U (250 µl)|
|Unit quantity||2500 U (250 µl)|
Restriction enzyme digestion of double-stranded DNA
AscI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)
|Expression System||An E. coli strain that carries the AscI gene from Arthrobacter sp. The AscI enzyme recognizes and cuts the sequence GG^CGCGCC.|
|Inactivation Protocol||80°C for 20 min|
|Reaction Definition||1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) Incubate at 37°C.|
Buffer 1: 10%, Buffer 2: 10%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes.
|Storage Buffer||10mM Tris pH (7.5), 50mM KCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol.|
Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked
There are no references for this product yet!