β-gal Adenovirus (E. coli)
| CAT.NO | UNIT | PRICE |
|---|---|---|
| 000197A | 1.0 ml | $245.00 |
| Description | This adenovirus is part of abm’s Adenoviral Expression System and can be used directly to transiently over-express your gene of interest in a wide range of host cells. This adenovirus can be used to amplify more adenovirus by transducing HEK293 cells. For enhanced transduction efficiency, the use of ViralPlus (G698) at 1:100 is recommended at the time of transduction. |
|---|---|
| SKU | 000197A |
| Unit quantity | 1.0 ml |
| Titer | >1x106pfu/mL |
| Accession Number | P00722 |
| Accession Number | P00722 |
| Format | Beta-galactosidase |
| System | Adenovirus |
| Species | E. coli |
| Promoter | CMV |
| Vector Map | pAdeno |
| Shipping Conditions | High titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Storage Condition | DMEM with 2.5% glycerol |
| Are your pPB protein expression vectors high or low copy number plasmids? | |
Our protein expression vectors are medium copy number plasmids and can be amplified using any standard miniprep or midi/maxi prep kits.
There is no standard protocol that fits all proteins, therefore recombinant protein expression will need to be optimized and determined experimentally. | |
| When the protein is expressed from this vector, which enzyme do I need to use to remove my tag? | |
The enzyme required to remove the tag (if possible) will depend upon the vector backbone corresponding to your product:
1) pPB-C-His: the His tag is NOT cleavable.
2) pPB-His-GST: the His-GST tag can be cleaved using TEV protease.
3) pPB-His-MBP: the His-MBP tag can be cleaved using TEV protease.
4) pPB-N-His: the His tag can be cleaved with Thrombin.
5) pPM-C-HA: the HA tag is NOT cleavable.
6) pPM-C-His: the His tag is NOT cleavable.
7) pPM-N-D-C-HA: the N-terminal D-tag can be cleaved using TEV protease. The C-terminal HA tag is NOT cleavable.
8) pPM-N-D-C-His: the N-terminal D-tag can be cleaved using TEV protease. The C-terminal His tag is NOT cleavable.
Please see the following page for further details:
https://www.abmgood.com/Protein-Vector.html | |
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