β-gal Adenovirus (E. coli)

CAT.NOUNITPRICE
000197A1.0 ml
$245.00

Specifications


DescriptionThis adenovirus is part of abm’s Adenoviral Expression System and can be used directly to transiently over-express your gene of interest in a wide range of host cells. This adenovirus can be used to amplify more adenovirus by transducing HEK293 cells. For enhanced transduction efficiency, the use of ViralPlus (G698) at 1:100 is recommended at the time of transduction.
SKU000197A
Unit quantity1.0 ml
Titer>1x106pfu/mL
Accession NumberP00722
Accession NumberP00722
FormatBeta-galactosidase
SystemAdenovirus
SpeciesE. coli
PromoterCMV
Vector MappAdeno
Shipping ConditionsHigh titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
Storage ConditionDMEM with 2.5% glycerol
Documents


Supporting Protocol
MSDS

    QC

      Other

        FAQs


        Are your pPB protein expression vectors high or low copy number plasmids?
        Our protein expression vectors are medium copy number plasmids and can be amplified using any standard miniprep or midi/maxi prep kits. There is no standard protocol that fits all proteins, therefore recombinant protein expression will need to be optimized and determined experimentally.
        When the protein is expressed from this vector, which enzyme do I need to use to remove my tag?
        The enzyme required to remove the tag (if possible) will depend upon the vector backbone corresponding to your product: 1) pPB-C-His: the His tag is NOT cleavable. 2) pPB-His-GST: the His-GST tag can be cleaved using TEV protease. 3) pPB-His-MBP: the His-MBP tag can be cleaved using TEV protease. 4) pPB-N-His: the His tag can be cleaved with Thrombin. 5) pPM-C-HA: the HA tag is NOT cleavable. 6) pPM-C-His: the His tag is NOT cleavable. 7) pPM-N-D-C-HA: the N-terminal D-tag can be cleaved using TEV protease. The C-terminal HA tag is NOT cleavable. 8) pPM-N-D-C-His: the N-terminal D-tag can be cleaved using TEV protease. The C-terminal His tag is NOT cleavable. Please see the following page for further details: https://www.abmgood.com/Protein-Vector.html
        References


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