Bacterial Alkaline Phosphatase

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E0921000 U (1 ml)
$60.00

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Bacterial Alkaline Phosphatase
Bacterial Alkaline Phosphatase

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$60.00

Summary
    Specifications


    DescriptionBacterial Alkaline Phosphatase is a non-specific phosphomonoesterase which catalyzes the removal of 5’ and 3’ phosphates from DNA and RNA.
    SKUE092
    Unit quantity1000 U (1 ml)
    Applications
    • Dephosphorylation of 5’ and 3’ ends of DNA and RNA.
    • Dephosphorylation of cloning vector DNA to prevent vector recircularization and reduce background.
    • Dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase.
    Concentration1000 U/ml
    FormatEnzyme supplied with a 10X Reaction Buffer. Incubate at 37°C.
    Unit DefinitionOne unit is defined as the amount of enzyme that hydrolyzes 1 μmol of p-Nitrophenyl Phosphate (PNPP) in a total reaction volume of 1 ml in 1 minute at 37°C.
    Storage Condition

    Store all components at -20°C.

    Storage Buffer12.5 mM Tris, 125 µM MgCl2, 12.5 µM ZnSO4, 50% Glycerol, pH 7.5.
    Note

    This product is distributed for laboratory research only.
    Caution: Not for diagnostic use.

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