Bestaq™ DNA Polymerase (PCR)

CAT.NOUNITPRICE
G4571,000 U (200 μl)
$115.00

Customize Bestaq™ DNA Polymerase (PCR)
1 x Bestaq, large   + $0.00
1 x 25mM MgSO4   + $0.00
4 x 5X PCR Buffer   + $0.00
1 x 5X GC Enhancer   + $0.00

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Bestaq™ DNA Polymerase (PCR)
Bestaq™ DNA Polymerase (PCR)

In stock

$115.00

Summary
    Specifications


    DescriptionLooking to improve your routine PCR? Bestaq™ DNA Polymerase is the new standard. With outstanding PCR yield, exceptional fidelity and high processivity, abm’s Bestaq™ DNA Polymerase is a versatile enzyme ideal for all PCR applications. With this superior enzyme, you can consolidate all PCR protocols and reactions into one efficient system. With abm’s Bestaq™ DNA Polymerase, you can expect • High-speed PCR without compromising accuracy • High-processivity to reduce reaction time by 70% • Robust and high yield across a wide range of templates • Efficient amplification of DNA templates up to 15kb
    SKUG457
    Applications• High-throughput PCR • Robust amplification of AT- and GC-Rich sequences • RACE • NGS Library construction
    Concentration5U/μl
    FormatEnzyme supplied with 5X buffer, MgSO4 and 5X GC Enhancer
    Note This product is ISO-13485 certified.
    Storage ConditionStore all components at -20 °C.
    Unit quantity1,000 U (200 μl)
    FAQs


    Does this enzyme have terminal transferase activity (i.e. adds an A at the end of the PCR product)?
    No, all high fidelity polymerases will not have this feature. If you would like to do T/A cloning on Bestaq PCR products, you can clean up the bestaq PCR reaction first, then mix the cleaned-up bestaq PCR product with Taq/PCR buffer/dNTP, and incubate it for 15-30 mins. The Taq will add the dA overhangs onto the bestaq PCR products.
    References


    1
    • MacKay, H. A.. "Identifying a native p-hydroxybenzoyl-CoA monolignol transferase gene in poplar (Doctoral dissertation, University of British Columbia)" : (2019).