Blastaq™ Green 2X qPCR MasterMix

CAT.NOUNITPRICE
G89250.0 ml
$795.00

Specifications


Description

SKU

G892

Concentration

2X

Storage Condition

Store at -20 °C.

Unit quantity

50.0 ml ( 1 x 50.0 ml)

Description

Guaranteed high-performance real-time PCR using abm’s Blastaq Green 2X qPCR MasterMix.

abm’s Blastaq Green 2X qPCR MasterMix provides all ingredients necessary for quantitative PCR in a premixed and optimized format. Provided with a separate vial of ROX reference dye, the internal passive dye level can be changed to fit different qPCR instrument models. abm’s Blastaq Green 2X qPCR MasterMix offers unparalleled performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers.

Key Features

  • Antibody hot-start technology provides superior specificity
  • Enable streamlined protocol in a simple reaction set-up
  • Allow accurate quantification of a variety of gene targets
  • Reduce pipetting steps to minimize the risk of contamination
  • Compatible with most real-time PCR instruments

SKUG892
Unit quantity50.0 ml
FAQs


What is the fidelity for TaqFast DNA Polymerase?
It is the same as regular Taq DNA Polymerase.
What is the Mg2+ concentration in the buffer?
Final concentration Mg2+ in reaction is 1.5mM.
Is it critical that the content is not mixed? What is the reason for not mixing. Would the reaction be affected if the content was mixed accidentally or shaken?
Yes, it is very important that the content cannot be mixed. This is a very standard procedures for direct PCR from blood samples. It is because blood samples contain a cocktail of PCR inhibitors and mixing the content would fully release these inhibitors to the reaction. The blood will remain and aggregate at the bottom of the PCR tube throughout the PCR, the "supernatant" will then be loaded onto a gel for analysis. The PCR reaction will very likely to fail if the content has been mixed. However, little turbulence may form during the dispense of blood sample but this wouldn't affect the PCR.
Is Bestaq DNA Polymerase compatible with Uracil?
Bestaq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.
Is Kodaq DNA Polymerase compatible with Uracil?
Kodaq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.
How can I optimize the reaction for multiplexing?
The polymerase and Mg amounts very often need to be optimized in multiplexing and this will need to be evaluated per case by the end user. Primer concentrations for each amplicon in multiplexing would also need to be optimized. The general rule would be lowering the primer concentrations of the strongly amplified targets and increasing the primer concentrations of the weakly amplified targets.
Can I vortex the reagents?
All abms PCR/qPCR/RT products can be vortexed, except for G915-2 (ExCellenCT Stop Solution).
References


27
  • Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans" DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
  • Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans" Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.
  • Ahn, CH et al. "Bacterial biofilm-community selection during autohydrogenotrophic reduction of nitrate and perchlorate in ion-exchange brine" Appl. Microbiol. Biotechnol. 81 (6):1169-1177 (2009). DOI: 10.1007/s00253-008-1797-3. PubMed: 19066883. Application: PCR.
  • Bhagwat, B et al. "An in vivo transient expression system can be applied for rapid and effective selection of artificial microRNA constructs for plant stable genetic transformation" J Genet Genomics 40 (5):261-270 (2013). DOI: 10.1016/j.jgg.2013.03.012. PubMed: 23706301. Application: PCR.
  • Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans" DNA Repair (Amst.) 10 (11):1174-1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
  • Lee, G et al. "Inhibition of in vitro tumor cell growth by RP215 monoclonal antibody and antibodies raised against its anti-idiotype antibodies" Cancer Immunol. Immunother. 59 (9):1347-1356 (2010). DOI: 10.1007/s00262-010-0864-7. PubMed: 20473495. Application: PCR.
  • Lee, G et al. "Cancer cell expressions of immunoglobulin heavy chains with unique carbohydrate-associated biomarker" Cancer Biomark 5 (4):177-188 (2009). DOI: 10.3233/CBM-2009-0102. PubMed: 19729827. Application: PCR.
  • Chaiyakul, M et al. "Cytotoxicity of ORF3 proteins from a nonpathogenic and a pathogenic porcine circovirus" J. Virol. 84 (21):11440-11447 (2010). DOI: 10.1128/JVI.01030-10. PubMed: 20810737. Application: PCR.
  • Lee, G et al. "Inhibition of in vitro tumor cell growth by RP215 monoclonal antibody and antibodies raised against its anti-idiotype antibodies" Cancer Immunol. Immunother. 59 (9):1347-1356 (2010). DOI: 10.1007/s00262-010-0864-7. PubMed: 20473495. Application: PCR.
  • Bhagwat, B et al. "An in vivo transient expression system can be applied for rapid and effective selection of artificial microRNA constructs for plant stable genetic transformation" J Genet Genomics 40 (5):261-270 (2013). DOI: 10.1016/j.jgg.2013.03.012. PubMed: 23706301. Application: PCR.
  • Chaiyakul, M et al. "Cytotoxicity of ORF3 proteins from a nonpathogenic and a pathogenic porcine circovirus" J. Virol. 84 (21):11440-11447 (2010). DOI: 10.1128/JVI.01030-10. PubMed: 20810737. Application: PCR.
  • Ahn, CH et al. "Bacterial biofilm-community selection during autohydrogenotrophic reduction of nitrate and perchlorate in ion-exchange brine" Appl. Microbiol. Biotechnol. 81 (6):1169-1177 (2009). DOI: 10.1007/s00253-008-1797-3. PubMed: 19066883. Application: PCR.
  • Chi, M et al. "Reduced polyphenol oxidase gene expression and enzymatic browning in potato (Solanum tuberosum L.) with artificial microRNAs" BMC Plant Biol. 14:62 (2014). DOI: 10.1186/1471-2229-14-62. PubMed: 24618103. Application: qRT-PCR.
  • Moazzen, H et al. "N-Acetylcysteine prevents congenital heart defects induced by pregestational diabetes" Cardiovasc Diabetol 13:46 (2014). DOI: 10.1186/1475- 2840-13-46. PubMed: 24533448. Application: qRT-PCR.
  • Lee, KH et al. "The Histone Demethylase PHF2 Promotes Fat Cell Differentiation as an Epigenetic Activator of Both C/EBPα and C/EBPδ" Mol Cells 37 (10):734-741 (2014). DOI: 10.14348/molcells.2014.0180. PubMed: 25266703. Application: qPCR.
  • Romic, S et al. "Does oestradiol attenuate the damaging effects of a fructose-rich diet on cardiac Akt/endothelial nitric oxide synthase signalling?" Br J Nutr 109(11):1940- 1948 (2013). DOI: 10.1017/S0007114512004114. Application: qPCR.
  • Chambers, AH et al. "Identification of a strawberry flavor gene candidate using an integrated genetic-genomic-analytical chemistry approach" BMC Genomics 15 (1):217 (2014). DOI: 10.1186/1471-2164-15-217. PubMed: 24742080. Application: qPCR.
  • Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy" Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: RT-PCR.
  • Kim, SJ et al. "Selective expression of transgene using hypoxia-inducible trans-splicing group I intron ribozyme" J Biotechnol. Pt A 22:7 (2014). DOI: 10.1016/j.jbiotec.2014.10.001. PubMed: 25312327. Application: RT-PCR.
  • Li, R et al. "Ultrafine particles from diesel vehicle emissions at different driving cycles induce differential vascular pro-inflammatory responses: implication of chemical components and NF-kappaB signaling" Part Fibre Toxicol 7:6 (2010). DOI: 10.1186/1743-8977-7-6. PubMed: 20307321. Application: RT-PCR.
  • Tang, Y et al. "Similar Gene Regulation Patterns for Growth Inhibition of Cancer Cells by RP215 or Anti-Antigen Receptors" J. Cancer Sci. Ther. 5:200-208 (2013). DOI: 10.4172/1948-5956.1000207. Application: RT-PCR.
  • Nagarajan, G et al. "Cloning and sequence analysis of IL-2, IL-4 and IFN-γ from Indian Dromedary camels (Camelus dromedarius)" Res. Vet. Sci. 92(3):420-426 (2012). DOI: 10.1016/j.rvsc.2011.03.028. Application: RT-PCR.
  • Ker, YB et al. "In vitro polyphenolics erythrocyte model and in vivo chicken embryo model revealed gallic acid to be a potential hemorrhage inducer: physicochemical action mechanisms" Chem. Res. Toxicol 26 (3):325-335 (2013). DOI: 10.1021/tx300456t. Application: PCR Products Viewing.
  • Safaee, AG et al. "Disease progression and patient survival are significantly influenced by BRAF protein expression in primary melanoma" Br J Dermatol 169(2):320-8 (2013). DOI: 10.1111/bjd.12351. PubMed: 23550516. Application: PCR Products Viewing.
  • Pyo, JS et al. "The prognostic relevance of psammoma bodies and ultrasonographic intratumoral calcifications in papillary thyroid carcinoma" World J Surg 37 (10):2330- 2335 (2013). DOI: 23716027. PubMed: 10.1007/s00268-013-2107-5. Application: PCR Products Viewing.
  • Nady, N et al. "Histone recognition by human malignant brain tumor domains" J Mol Biol 423 (5):702-718 (2012). DOI: 10.1016/j.jmb.2012.08.022.. Application: PCR Products Viewing.
  • Ish-Shalom, S et al. "Transformation of Botrytis cinerea by direct hyphal blasting or by wound-mediated transformation of sclerotia" BMC Microbiol 11:266 (2011). DOI: 10.1186/1471-2180-11-266. Application: PCR Products Viewing.