Cas9 Nuclease Non-Viral Vector

CAT.NOUNITPRICE
K09510 μg
$85.00

Specifications


Description

This Cas9 nuclease can be used with a target-specific sgRNA to bind DNA and create a double-stranded cut. It is provided in a non-viral vector (plasmid) for quick and easy transfection. Transfection will result in transient expression of Cas9, leading to reduced chances of off-target editing. The Cas9 provided in this non-viral vector is spCas9 from Streptococcus pyogenes.

SKUK095
SystemNon-Viral Vector
Product Applications
  • Direct non-viral plasmid transfection for immediate expression.
  • Transient sgRNA expression reduces the chance of off-target effects.
Vector Map

pNV-EF1a-Cas9-2A-GFP

Unit quantity10 μg
PromoterEF1a
Cas TypeNuclease
Cas OriginspCas9
Cas9 ExpressionConstitutive Cas9
Note

abm's sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNAs for purposes other than genome editing (e.g. CRISPRa). For CRISPR-Cas9 system applications in genome regulation, please see our comprehensive collection of Cas9 mutant proteins (Nickase, Null mutant).

Shipping Conditions

Shipped at ambient temperature.

Bacterial SelectionAmpicillin
FormatPlasmid
AppearanceLiquid
Quality ControlRestriction Enzyme Digest and Sequencing
Shelf Life1 year (when at -20°C or below in a non-frost free freezer)
CautionThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Documents


Supporting Protocol

    MSDS

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