Cas9 Nuclease Protein
|K008||8.0µg (50pmol/ 50µL)|
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.
|Cas Protein Marker||No GFP|
|Alias||spCas9, spyCas9, CRISPR-associated endonuclease Cas9 from Streptococcus pyogenes|
|Unit quantity||8.0µg (50pmol/ 50µL)|
|Product Concentration||1000 nM, 160 µg/ml|
|Format||Enzyme supplied with 10X Reaction Buffer|
|Storage Buffer||10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.|
|Storage||Store all components at -20°C.|
|Caution||This product is distributed for laboratory research only. Caution: Not for diagnostic use.|
|Does K008 contain a HIS-tag?|
Yes, it does contain a His-tag.
|Does this cas9 nuclease protein contain a Nuclear Localization Signal?|
No, this Cas9 nuclease protein does not have a Nuclear Localization Signal and therefore represents the native form. If NLS is desired, we would recommend our Cas9 Nuclease NLS Protein (Cat. No. K030, K130). A full list of our Cas9 products can be found here:
|What is the in vitro digestion of DNA protocol with Cas9 Nucleases?|
The substrate DNA: sgRNA : Cas9 molar ratio must be kept at 1:10:10 for highest efficiency.
- Nelson, C. E., Wu, Y., Gemberling, M. P., Oliver, M. L., Waller, M. A., Bohning, J. D., … Gersbach, C. A. "Long-term evaluation of AAV-CRISPR genome editing for Duchenne muscular dystrophy" Nature Medicine 25(3):427–432 (2019). DOI: 10.1038/s41591-019-0344-3.