DNA and RNA Mesophilic Polymerases
abm offers an all inclusive range of mesophilic polymerases that enable the preparation of RNA and DNA for subsequent application(s) in next generation whole genome sequencing and library construction.
- Sku: G469
Unit: 500 U (100 μl)E.coli DNA Polymerase I is a DNA-dependent DNA polymerase with intrinsic 3’→5’ and 5’→3’ exonuclease activities. Nick translation is facilitated by this enzyme due to the removal of nucleotides ahead of the growing chain via 5´→3´...
- Sku: E041
Unit: 100 ul (5000 U)T7 RNA Polymerase is a DNA dependent RNA polymerase that catalyzes the synthesis of RNA in the 5´→ 3´ direction only in the presence of its cognate T7 phage promoter sequence. T7 RNA Polymerase has high specificity for the T7 phage promoter and...
- Sku: E038
Unit: 500 U (100 μl)Klenow Fragment (3’→5’ Exo-) is the large fragment of E. coli DNA Polymerase I which retains polymerase activity but lacks 5’→3’ exonuclease activity and has mutations that effectively abolish the inherent 3’→5’ exonuclease...
- Sku: E017
Unit: 100 U (100 μl)Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP)...
- Sku: E014
Unit: 1000 U (100 μl)Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification...
- Sku: E013
Unit: 500 U (100 μl)DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I (abm Cat. No. G469). The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5’→3’...
- Sku: E009
Unit: 25 ReactionsThe DNA dA-Tailing Kit efficiently adds a non-template dAMP (dA) to the 3’ end of a blunt-ended DNA fragment. This incorporated 3’-dA prevents concatemer formation and prepares the DNA fragment for subsequent ligation of adaptors or cloning...
- Sku: E005
Unit: 500 U (100 μl)T4 DNA Polymerase catalyzes the 5’→3’ synthesis of DNA from a single stranded, primed DNA template. This high fidelity enzyme also has potent 3’→5’ exonuclease activity but lacks 5’→3’ exonuclease function. T4 DNA Polymerase is the...