Nucleases

abm offers a range of nucleases that enable the preparation of RNA and DNA for subsequent application(s) in next generation whole genome sequencing and library construction.

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  1. Price: $95.00
    Unit: 200 U (100 μl)
    Deoxyribonuclease I (DNase I; EC 3.1.21.1) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide...


  2. Price: $85.00
    Unit: 2000 U (1.0 ml)
    Deoxyribonuclease I (DNase I; EC 3.1.21.1) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide...


  3. Price: $55.00
    Unit: 1000 U (100 µl)
    T5 exonuclease catalyzes the degradation of nucleotides either from the 5’ termini or at nicks of linear or circular dsDNA in a 5’ to 3’ direction. This exonuclease also exhibits ssDNA endonuclease activity in the presence of magnesium ions,...


  4. Price: $220.00
    Unit: 500 U (50 μl)
    RNase R is an E. coli exoribonuclease which exhibits 3'-to-5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3’ overhangs (less than seven...


  5. Price: $50.00
    Unit: 1000 U (100 μl)
    Nt.BstNBI (Bst Nickase) is a nicking endonuclease from Bacillus stearothermophilus that cleaves one DNA strand of a double-stranded DNA substrate. This site-specific endonuclease cleaves a single strand of DNA four base pairs away and 3’ of its...


  6. Price: $125.00
    Unit: 1000 U (100 μl)
    T7 Endonuclease I recognizes and cleaves mismatched DNA, heteroduplex DNA, cruciform DNA, Holliday structures and/or junctions, as well as nicked double-stranded DNA. This enzyme has a preference for single stranded over double stranded DNA with...


  7. Price: $85.00
    Unit: 100 U (100 μl)
    RNase III, E.coli is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3′-overhangs with 5' phosphate and 3' hydroxyl termini.


  8. Price: $25.00
    Unit: 500 U (100 μl)
    RNase H (Ribonuclease H), E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA strands in RNA-DNA hybrids. This enzyme cleaves the 3'-O-P-bond of RNA and generates 3' hydroxyl and 5' phosphate products. RNase...


  9. Price: $35.00
    Unit: 10 000 U (100 μl)
    Exonuclease III, E.coli digests duplex DNA in the 3′→5′ direction from nicked DNA, blunt end DNA, 3′-recessed ends, or 3′-overhangs of less than four bases, and yields nucleoside 5′-phosphates. The DNA degradation proceeds at a uniform...


  10. Price: $15.00
    Unit: 2000 U (100 μl)
    Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3’→5’ direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the...

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