PCR

As an industry-leading and global biotech company, abm offers a wide range of ISO13485 certified PCR, RT-PCR and qPCR enzyme products suitable for diagnostic use.

  • PCR: Over 10 strategically engineered DNA polymerases are available, offered in enzyme-only and 2X PCR MasterMix formats.
  • RT: Two supreme OneScript® Reverse Transcriptases offered as All-In-One RT MasterMix, RT SuperMix, One-Step cDNA Synthesis Kits, and One-Step qRT-PCR Kits.
  • qPCR: Dye-based 2X and 5X qPCR MasterMixes suitable for standard and fast cycling conditions, as well as specially formulated MasterMixes suitable for miRNA and long amplicons. TaqProbe-based 2X and 5X qPCR MasterMixes are also available.
  • Nucleic Acid Staining: Developed to replace toxic Ethidium Bromide, SafeView™ products are a new class of nucleic acid stains for the visualization of double-stranded DNA, single-stranded DNA, and RNA in both agarose and polyacrylamide gels.
  • Specialized Kits: Real-time qPCR detection kits of viral particles for recombinant lentivirus, retrovirus and AAV, as well as PCR-based mycoplasma detection Kit for cell culture applications. Crude sample preparation kits for tissues, plants, blood or cell samples are also available.
"I ordered some RT MasterMix and qPCR MasterMix. Both of them are very good, especially the BrightGreen qPCR Mastermix, which gives a very good signal and smaller cycle number than Sybrgreen. The price is cheaper as well. We will order more PCR reagents from ABM in the future."

Xiaoyun Tang, University of Alberta, BrightGreen 2X qPCR MasterMix-ROX and 5X All-In-One RT MasterMix

"I am very impressed with abm's RT MasterMix. It was very easy to use and the reaction time was very short."

Boram Ham, McGill University, Reverse Transcriptase MasterMix

Resources

  • Introduction to PCR
    Introduction to PCR
    Polymerase Chain Reaction Knowledge Base
  • DNA Polymerase Variation
    DNA Polymerase Variation
    Polymerase Chain Reaction Knowledge Base
  • Real-Time PCR/Quantitative PCR (qPCR)
    Real-Time PCR/Quantitative PCR (qPCR)
    Polymerase Chain Reaction Knowledge Base
  • Reverse Transcription (RT-PCR)
    Reverse Transcription (RT-PCR)
    Polymerase Chain Reaction Knowledge Base
  • Variations to System
    Variations to System
    Polymerase Chain Reaction Knowledge Base
  • PCR RT-PCR qPCR Application Handbook
    PCR RT-PCR qPCR Application Handbook
    Application Handbook
  • PCR Product Brochure and Performance Data
    PCR Product Brochure and Performance Data
    PCR Brochure
  • qRT-PCR Product Brochure and Performance Data
    qRT-PCR Product Brochure and Performance Data
    qRT-PCR Brochure

Top Publications

01

Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome

Wood, L. W., et al
Scientific Reports (2016) 6(19857)


doi: 10.1038/srep19857

02

Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid binding protein

Ly, D., et al
Scientific Reports (2016) 6(35651)


doi: 10.1038/srep35751

03

MICU1 regulation of mitochondrial Ca(2+) uptake dictates survival and tissue regeneration

Antony, A., et al
Nature Communications (2016) 9(7):10955-65


doi: 10.1038/ncomms10955

Testimonials

I am very satisfied this product. It is easy to use and accurate. That's why I keep using them to check lentivirus titer. Thank you abm for developing this kit which makes my research easier.

Xinping Huang
Emory University

qPCR Lentivirus Titer Kit

I am a fan of abm: great product quality, great price, great rewards and great experience.

Dr. Vincent Emond
Centre de recherche du CHU de Québec CHUL

Safe-Green, Opti-Protein XL ladders and Safe-Green 100bp Opti-DNA ladders

I have recently switched to using this product and am very happy with the results. Bestaq is reliable, fast, easy to use, and competitively priced. I will be using Bestaq for the reminder of my cloning throughout my studies.

Heather MacKay
The University of British Columbia

Bestaq DNA Polymerase

Promotions

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  1. Sku: G892
    Price: $495.00
    Unit: 25.0 ml
    Guaranteed high-performance real-time PCR using abm’s Blastaq™ Green 2X qPCR MasterMix. abm’s Blastaq™ Green 2X qPCR MasterMix provides all ingredients necessary for quantitative PCR in a premixed and optimized format. Provided with a...


  2. Sku: G893
    Price: $795.00
    Unit: 50.0 ml
    Guaranteed high-performance real-time PCR using abm’s Blastaq™ Green 2X qPCR MasterMix. abm’s Blastaq™ Green 2X qPCR MasterMix provides all ingredients necessary for quantitative PCR in a premixed and optimized format. Provided with a...


  3. Sku: G891
    Price: $125.00
    Unit: 5.0 ml (4 x 1.25 ml)
    Guaranteed high-performance real-time PCR using abm’s Blastaq™ Green 2X qPCR MasterMix. abm’s Blastaq™ Green 2X qPCR MasterMix provides all ingredients necessary for quantitative PCR in a premixed and optimized format. Provided with a...


  4. Sku: G894
    Price: $375.00
    Unit: 2000 U
    Streamline your PCR with abm’s Blastaq™ DNA Polymerase (5 U/µl). abm’s Blastaq™ DNA Polymerase (5 U/µl) is a next generation Taq Polymerase strategically-engineered to have incredibly fast extension rates and very robust performance.  ...


  5. Sku: G895
    Price: $245.00
    Unit: 10.0 ml (800 Rxns)
    Streamline your PCR with abm’s 2X Blastaq™ PCR Taq MasterMix. abm’s 2X Blastaq™ PCR Taq MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Blastaq™ DNA Polymerase is...


  6. Sku: G896
    Price: $475.00
    Unit: 400 rxn (200 µl)
    MegaFi™ Fidelity DNA Polymerase offers incredible sensitivity and speed, even with challenging templates, including GC-/AT-rich and long amplicons. MegaFi™ Fidelity DNA polymerase sets new standards for sensitive, robust, and high-fidelity...


  7. Sku: G897
    Price: $295.00
    Unit: 800 rxn (10.0 ml)
    Streamline your PCR with abm’s MegaFi™ Fidelity 2X PCR MasterMix. abm’s MegaFi™ Fidelity 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. This...


  8. Sku: PCR-Sure-configurable
    Price: $85.00


  9. Sku: SensTaq-configurable
    Price: $40.00


  10. Sku: Long-Range-configurable
    Price: $30.00

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