Screen It™ CRISPR Cas9 Cleavage Detection Kit

Cat. No.
G990
Unit
100 Reactions
Price
$1500.00 $750.00
Cat. No. G990
Name Screen It™ CRISPR Cas9 Cleavage Detection Kit
Unit 100 Reactions
Category Molecular Biology Enzymes and Kits
Description

abm’s Screen It™ CRISPR Cas9 Cleavage Detection Kit is a robust and precise RNA-dependent assay used to identify successful insertions and/or deletions (indels) of genomic DNA following a CRISPR-Cas9 experiment. CRISPR-Cas9 can introduce indels on either one or both copies of a given mammalian gene. abm’s Screen It™ CRISPR Cas9 Cleavage Detection Kit can easily identify if a given clone is monoallelic (has mutations in one copy), biallelic (both copies mutated) or is unchanged wild-type which is considered a major screening advantage over the standard T7E1 Surveyor Assay. This kit uses PCR to first amplify the sgRNA target site from CRISPR edited cells; this amplicon is subsequently cleaved by a ribonucleoprotein (RNP) complex which generates a unique pattern that can be easily resolved on agarose gel and interpreted by the user. Using this kit after gene editing saves time and energy from the arduous screening process and provides clear genotyping results.  

Product Component Quantity
Cell Lysis Buffer  1.25 ml
Protein Degrader 100 µl
Scaffold Template and Primer Mix 100 μl
2X sgRNA Synthesis Buffer 100 μl
sgRNA Synthesis Enzyme Mix 50 μl
sgRNA Control Oligo 20 µl
Wild Type Control Primer and Template Mix 20 µl
Monoallelic Control Primer and Template Mix 20 µl
Biallelic Control Primer and Template Mix 20 µl
RNP Degrader 100 µl
spCas9 Nuclease Protein 250 µl
10X Cas9 Reaction Buffer 1.25 ml
MegaFi™ Pro Fidelity 2X MasterMix 2 x 1.25 ml

Additional Materials Required
Target-specific Primers (abm offers sgRNA PCR Primer Pair Design & Synthesis Service, Cat. No. C336)
sgRNA Target-specific Oligo (abm offers sgRNA Target-Specific Oligo Design & Synthesis Service, Cat. No. C337)
CRISPR-edited Cells        
Wild-type Cells 
Nuclease-free Water

Storage Condition Store all components at -20°C.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G990
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