Screen It™ CRISPR Cas9 Cleavage Detection Kit

abm’s Screen It™ CRISPR Cas9 Cleavage Detection Kit is a robust and precise RNA-dependent assay used to identify successful insertions and/or deletions (indels) of genomic DNA following a CRISPR-Cas9 experiment. CRISPR-Cas9 can introduce indels on either one or both copies of a given mammalian gene. abm’s Screen It™ CRISPR Cas9 Cleavage Detection Kit can easily identify if a given clone is monoallelic (has mutations in one copy), biallelic (both copies mutated) or is unchanged wild-type which is considered a major screening advantage over the standard T7E1 Surveyor Assay. This kit uses PCR to first amplify the sgRNA target site from CRISPR edited cells; this amplicon is subsequently cleaved by a ribonucleoprotein (RNP) complex which generates a unique pattern that can be easily resolved on agarose gel and interpreted by the user. Using this kit after gene editing saves time and energy from the arduous screening process and provides clear genotyping results.  

Additional Materials Required
Target-specific Primers (abm offers sgRNA PCR Primer Pair Design & Synthesis Service, Cat. No. C336)
sgRNA Target-specific Oligo (abm offers sgRNA Target-Specific Oligo Design & Synthesis Service, Cat. No. C337)
CRISPR-edited Cells        
Wild-type Cells 
Nuclease-free Water