Bst DNA Polymerase

Cat. No.
G880
Unit
200 rxn
Price
$115.00
Cat. No. G880
Name Bst DNA Polymerase
Unit 200 rxn
Category Molecular Biology Enzymes and Kits
Description

abm’s Bst DNA Polymerase for Loop Mediated Isothermal Amplification (LAMP) offers a rapid and highly specific method for isothermal applications, including low copy detection of a target gene from a DNA template. In addition to its high specificity, Bst DNA Polymerase offers easy, reliable and reproducible results in optimized conditions.

This robust polymerase has exceptional specificity and can extend the time of potential spurious amplification in your No template control under optimized conditions allowing you to feel more confident in your results. 

Product Features:

  • Rapid and highly specific method for isothermal applications
  • Low copy detection of a target gene from a DNA template
  • Earn 2X the rewards points

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ISO 13485:2016 MDSAP Certified

ISO 13485:2016 MDSAP Certified
Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

 

Product Component Quantity
Bst DNA Polymerase 200 rxn (200µl)
10X Bst LAMP Reaction Buffer 1.0 ml
Nuclease-Free H2O 1.0ml

 

Tips: recommend to optimize the reactions for each primer set and determining the ideal time for the reaction to be ran for.

  1. Determine optimal temperature. Using a temperature gradient for a set time, evaluate the quality of bands and if they follow the expected. Temperatures can range from 55°C to 72°C for reaction runs. 
  2. Determine the optimal reaction time. High or low copy number of amount of template input will influence the time it takes to see a positive reaction. Recommend to start with 15 minute intervals. Check on your NTC as with longer reaction times the control is susceptible to false positive. Confirm results of amplification of the NTC by running product on agarose gel and evaluate the banding pattern.

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Storage Condition Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G880
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