Ultra-Pure BlasTaq™ 2X qPCR MasterMix
|Name||Ultra-Pure BlasTaq™ 2X qPCR MasterMix|
|Unit||125 rxn (1.25 ml)|
Ultra-Pure BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, Ultra-Pure BlasTaq™ DNA Polymerase, providing for rapid extension rates and robust performance.
With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. The enzyme is subject to a rigorous multi-step purification protocol using physical, chemical, and enzymatic methods for maximum removal of contaminating genomic DNA.
Ultra-Pure BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with Ultra-Pure BlasTaq™ can be used with TA cloning vectors. The MasterMix contains dye comparable to SYBR Green™ and EvaGreen™.
Store at -20 °C. This product is stable for 2 years from the date of shipping if stored and handled properly.
Ultra-Pure BlasTaq™ 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.
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