Dulbecco's Modified Eagle Medium (DMEM), High Glucose

Cat. No.
500 ml
Cat. No. TM500
Name Dulbecco's Modified Eagle Medium (DMEM), High Glucose

DMEM, or Dulbecco's Modified Eagle Medium, is basal medium widely used for the growth of many mammalian cells, such as primary fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells, HeLa, 293, Cos-7, and PC-12 cell lines. This formulation contains high glucose. Supplementation is required, most commonly with 10% fetal bovine serum (FBS) for cell culture use, and requires a 5-10% CO2 environment to maintain physiological pH.

DMEM is modified as follows:

• High Glucose
• L-Glutamine
• Sodium Pyruvate
• Sodium Bicarbonate (3.7 g/L)
• Phenol Red


Special media modification is available as a Custom Media Service, please contact a technical representative at [email protected] for more details.

Unit 500 ml
Sterility Pass
Appearance Solution

Each lot has been fully tested and optimized for its ability to support in vitro cell culture. The testing parameters include: adherence rate (where applicable), typical morphology, and metabolism of the tested cells. Additionally, the media has been tested for sterility, pH, osmolality and endotoxin levels.

Storage Condition Store at 2-8°C. Protect from light.

For research use only and is not intended for therapeutic or diagnostic applications.


abm products are intended for laboratory research purposes only, unless noted otherwise. They are not intended for use in humans. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. TM500
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