DMEM/F12 Medium

Cat. No.
500 ml
Cat. No. TM503
Name DMEM/F12 Medium

DMEM/F12, or Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12), is basal medium widely used for the growth of many mammalian cells, such as MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. A 1:1 mixture of DMEM and Ham's F-12. Supplementation is required, most commonly with 10% fetal bovine serum (FBS) for cell culture use, and requires a 5-10% CO2 environment to maintain physiological pH.

DMEM/F12 is modified as follows:

• L-Glutamine
• Sodium Bicarbonate (2.438 g/L)
• Phenol Red


Special media modification is available as a Custom Media Service, please contact a technical representative at [email protected] for more details.

Unit 500 ml
Sterility Pass
Appearance Solution
Storage Condition Store at 2-8°C. Protect from light.

For research use only and is not intended for therapeutic or diagnostic applications.


abm products are intended for laboratory research purposes only, unless noted otherwise. They are not intended for use in humans. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. TM503
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