Retro-SV40 Retrovirus
Cat. No.
G212
Unit
5 x 50 µl
Price
$1025.00
| Cat. No. | G212 |
| Name | Retro-SV40 Retrovirus |
| Unit | 5 x 50 µl |
| Unpacking and Storage Instructions |
-80°C |
| Description |
Retrovirus expressing SV40 large and small T antigens (108IU/ml) for cell immortalization. Often used to immortalize difficult to transduce cells like epithelial cells and lymphocytes (B cells, T cells, etc...). |
| Vector Map | pRetro-E2 SV40 |
| Note |
*For for-profit organizations and corporations, the purchase price is 1.5 times the listing price. |
| Caution |
NOT FOR RESALE without prior written consent of abm. This product is distributed for laboratory research only. Caution: Not for diagnostic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G212 |
Print & Download Datasheet
| What are the correct concentration units for each recombinant viral particle? | |
|
For lentiviruses and retroviruses, they are measured in CFU/ml (colony-forming units per millilitre). Transduction with lentiviruses and retroviruses can cause the formation of colonies, which can be quantified for concentration. For AAV the titer is measured as genome copies per mL (GC/mL). Adenoviruses are measured as PFU/ml (plaque-forming units per millilitre). Transduction with adenoviruses will kill packaging cells, forming plaques in the process for quantification. The concentration for all three types of viruses can also be classified as IU/ml (Infectious Units/ml). Ultimately, the units refers to the viral particles and different units reflect the different assays involved.
|
| What do I use to check if my cells were successfully immortalized by the SV40 agent? | |
|
We have an SV40 T antibody that can be used for the western blot analysis. The catalog number is G202.
Otherwise, a qPCR primer can be designed on the SV40 gene for qPCR analysis. The sequence can be found in the link below:
http://www.abmgood.com/pLenti%20SV40-Vector-Location-Map.html
|
| What are the primers to use for SV40 identification? | |
|
SV40 Forward Primer Sequence
5’ ACTGAGGGGCCTGAAATGA
SV40 Reverse Primer Sequence
5’ GACTCAGGGCATGAAACAGG
These are qPCR primers and the band size is 61 bp.
|
| What advantages / disadvantages exist between the Lenti-SV40, -SV40T, and SV40T+t vectors? | |
|
There are simply differences in the content of all vectors due to customer demand for variety. Lenti-SV40 will contain the whole SV40 gene, -SV40T, the large T Antigen only, and -SV40T&t the large and small T antigens only.
It is up to the end user to decide which vectors will best suit their project, however we have successfully used Lenti-SV40 (whole gene) in a wide range of immortalization projects.
|
| What is the accession number for the SV40? | |
|
The SV40 covers the entire genome and the accession number is J02400.1. You can use this information to design primers for conventional PCR as well.
|
| How long after transduction can the infection efficiency be observed? | |
|
You can observe transduction efficiency from 48 hours up to 5 days after infection.
|
| What are the primers to use for SV40T and SV40T tsA58 detection? | |
|
PCR primers:
SV40T Forward Primer Sequence
5’ AGCCTGTAGAACCAAACATT 3'
SV40T Reverse Primer Sequence
5’ CTGCTGACTCTCAACATTCT 3'
The two primers should amplify the region between 3677-4468bp, giving a 792bp fragment.
|
| What is the sequence of the SV40 large T antigen? | |
|
This information can be accessed on this page by clicking on "pLenti-SV40-T" under vector map. The Large T antigen is at position 5079-5927.
|
| Where is the SV40T tsA58 gene sequence? | |
|
The SV40T tsA58 gene is located between 3138-5264bp, with the Alanine-to-Valine mutation at amino acid 438.
|
|
What PCR primers can I use for SV40 detection?
|
|
|
PCR primers:
SV40T Forward Primer Sequence
5’ AGCCTGTAGAACCAAACATT 3'
SV40T Reverse Primer Sequence
5’ CTGCTGACTCTCAACATTCT 3'
Expected band size: 792bp
|
| What qPCR primers can I use for SV40 detection? | |
|
SV40 Forward Primer Sequence
TTCCCTGACCTGAAGGCAAATC
SV40 Reverse Primer Sequence
GGCTGAACTTTGAGCTAGGAGTAG
|
- Wu, L et al. " Immortalized Mouse Floxed Bmp2 Dental Papilla Mesenchymal Cell Lines Preserve Odontoblastic Phenotype and Respond to BMP2. J. Cell" Physiol 225:132-139 (2010). DOI: 10.1002/jcp.22204. Application: Immortalization.
- Olyslaegers, D et al. "Generation and characterization of feline arterial and genous endothelial cell lines for the study of the vascular endothelium" BMC Veterinary Research 9:170 (2013). DOI: 10.1186/1746-6148-9-170. Application: Immortalization.
- Desmarets, L et al. "Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline" Veterinary Research 44:71 (2013). DOI: 10.1186/1297-9716-44-71. Application: Immortalization.
- Narendra, DP;Jin, SM;Tanaka, A;Suen, DF;Gautier, CA;Shen, J;Cookson, MR;Youle, RJ;, et al. "PINK1 is selectively stabilized on impaired mitochondria to activate Parkin" PLoS Biol. 8-1:e1000298 (2010). PubMed: 20126261.
- Balducci, L et al. "Immortalization of human adipose-derived stromal cells: production of cell lines with high growth rate, mesenchymal marker expression and capability to secrete high levels of angiogenic factors" Stem Cell Research & Therapy 3:63 (2014). DOI: 10.1186/scrt452. PubMed: 24887516. Application: Immortalization.
- Wong, SY et al. "Primary cilia can both mediate and suppress Hedgehog pathway–dependent tumorigenesis" Nat. Med. 9:1055 - 1061 (2009). DOI: doi:10.1038/nm.2011. PubMed: 19701205. Application: Immortalization.
- Wang, ZY et al. "Immortalized porcine liver sinusoidal endothelial cells: an in vitro model of xenotransplantation-induced thrombocytopenia" Xenotransplantation 19:249-55 (2012). PubMed: 22909138.
- Chen, Q et al. "Pharmacological inhibition of S-nitrosoglutathione reductase improves endothelial vasodilatory function in rats in vivo" J. Appl. Physiol. 6:752-60 (2013). DOI: 10.1152/japplphysiol.01302.2012. PubMed: 23349456. Application: Immortalization.
- Zhao, Y et al. "microRNA response elements-regulated TRAIL expression shows specific survival-suppressing activity on bladder cancer." J Exp Clin Cancer Res 32(1):10 (2013). DOI: 10.1186/1756-9966-32-10. PubMed: 23442927. Application: Immortalization.
- Liu, J et al. "Oleanolic acid inhibits proliferation and invasiveness of Kras-transformed cells via autophagy" J Nutr Biochem 25(11):1154-1160 (2014). DOI: 10.1016/j.jnutbio.2014.06.006. PubMed: 25172632.
- Nauwynck, H.J. et al. "Generation and Characterization of feline arterial and cenous endotheilial cell lines for the study of the vascular endothelium " Biomed Central 9: (2013). DOI: 10.1186/1746-6148-9-170. Application: Gene Delivery.
- Laval, et al. "Equine Herpesvirus Type 1 Enhances Viral Replication in CD172a+ Monocytic Cells upon Adhesion to Endothelial Cells" Journal of Virology 21:10912-10923 (2015). DOI: 10.1128/JVI.01589-15.
- Liu, et al. "Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal and Osteoblast Cell Lines Retain Their Primary Characteristics" Journal of Cellular Physiology 11:2581-2587 (2015). DOI: 10.1002/jcp.25008.
- Coleman, S et al. "A Homolog Pentameric Complex Dictates Viral Epithelial Tropism, Pathogenicity and Congenital Infection Rate in Guinea Pig Cytomegalovirus" PLoS Pathog 12.7:e1005755 (2016). DOI: 10.1371/journal.ppat.1005755. Application: Immortalization.
- Jester, JV et al. "PPARγ Regulates Mouse Meibocyte Differentiation and Lipid Synthesis" The Ocular Surface 14.4:484-494 (2016). DOI: 10.1016/j.jtos.2016.08.001. Application: Immortalization.
- Wang, ZY et al. "Immunogenicity of Renal Microvascular Endothelial Cells From Genetically Modified Pigs" Transplantation 100.3:533-537 (2016). DOI: 10.1097/TP.0000000000001070. Application: Immortalization.
This product has no review yet.