Chicken iPSC

CAT.NOUNITPRICE
T25021x106 cells / 1.0 ml
$2,850.00

Specifications


DescriptionDerived from chicken embryonic fibroblasts after lentiviral transduction with human stem cell genes; the Chicken iPSC line was developed as a serum-free; suspension cell line for use as an alternative to egg-based methods in viral vaccine research. Eliminating the need for trypsinization and anchorage support; suspension cells are preferable for vaccine production. In addition to decreasing overall production cost; serum-free cell expansion eliminates a potential source for viral or prion contamination; an important consideration for work in vaccine production.
SKUT2502
SpeciesChicken (Galline)
Tissue/Organ/Organ SystemEmbryo
Applications

For Research Use Only

Seeding Density7 x 105 cells/ml
Population Doubling TimeApproximately 21 hours
Cell TypeReady-to-Use Stem Cells
Growth PropertiesSuspension
Expression Profile

lentiviral transduction utilizing the viPC kit (Thermo Scientific) with viruses containing the human stem cell genes POU5F1; NANOG; SOX2; LIN28; KLF4 and C-MYC under the promoter of human elongation factor-1 alpha (EF1a)

Subculture Protocol

Plate at 7 x 105 cells/mL and incubate on a shaker agitated at 50-100 rpm

Unit quantity1x106 cells / 1.0 ml
QC

1) PCR

Documents


Supporting Protocol

    MSDS

      QC

        Other

          FAQs


          There are no FAQs for this product yet!
          References


          There are no references for this product yet!