|T2502||1x106 cells / 1.0 ml|
|Description||Derived from chicken embryonic fibroblasts after lentiviral transduction with human stem cell genes; the Chicken iPSC line was developed as a serum-free; suspension cell line for use as an alternative to egg-based methods in viral vaccine research. Eliminating the need for trypsinization and anchorage support; suspension cells are preferable for vaccine production. In addition to decreasing overall production cost; serum-free cell expansion eliminates a potential source for viral or prion contamination; an important consideration for work in vaccine production.|
|Applications||For Research Use Only|
|Seeding Density||7 x 105 cells/ml|
|Population Doubling Time||Approximately 21 hours|
|Cell Type||Ready-to-Use Stem Cells|
|Expression Profile||lentiviral transduction utilizing the viPC kit (Thermo Scientific) with viruses containing the human stem cell genes POU5F1; NANOG; SOX2; LIN28; KLF4 and C-MYC under the promoter of human elongation factor-1 alpha (EF1a)|
|Subculture Protocol||Plate at 7 x 105 cells/mL and incubate on a shaker agitated at 50-100 rpm|
|Unit quantity||1x106 cells / 1.0 ml|
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