CMV Promoter Thomson Factors Lentivirus Set
|G353||5 x 200 μl|
|Description||Five Lenti-III-CMV viruses, expressing the Thomson iPSC factor set (Oct4, Sox2, Nanog and Lin28) and a GFP control. Purified ready-to-use high titer lentivirus at 1x107 cfu/mL|
|Species||Human (H. sapiens)|
|iPSCS Factor Type||Lentivirus|
|iPSC Factor||Thomson Protocol|
|Gene Name||Oct4, Sox2, Nanog, Lin28 and GFP control virus.|
|Vector Map||Refer to Individual Virus Pages|
|Shipping Conditions||On Dry Ice|
|Caution||For Research Use Only, not for therapeutic or diagnostic purposes.|
|Function||Each individual recombinant lentivirus is provided as a VSV-G pseudotyped and concentrated virus stock capable of infecting both dividing and non-dividing cells. The expression of the four transcription factors provided in this set in combinantion with each other has been shown to reprogram adult human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs).|
|Unit quantity||5 x 200 μl|
|QC||Viral titer (IU/ml) is determined by quantitative RT-PCR of viral stock preparation. Transcription factor protein expression are verified by immunocytochemistry and Western Blot analysis of transduced 293 cells. All of ABM's viral preparations are tested to be free of bacteria and other microbials.|
|What is the transfection efficiency for the minicircles?|
Minicircles have lower transfection efficiency than infection with viral particles. However, minicircles are safer for the cells because they do not have additional non-coding components.
|What volume and concentration is the minicircle DNA provided at?|
Minicircle DNA products are provided as 100ug DNA at 0.5ug/ul concentration (200 ul total volume).
|What is the difference in promoters between LV028873 and LV028874?|
LV028873 PL-SIN-EOS-C(3+)-EiP Lentivirus Includes C(3+), trimer of CR4 enhancer LV028874 PL-SIN-EOS-S(4+)-EiP Lentivirus Includes S(4+), tetramer of SRR2 enhancer
|Do you offer a protocol for iPSC generation using episomal plasmids?|
There are several papers referencing a number of methods of iPSC generation, these have been included below for more information. Further to this, we can offer no standard protocol for using these plasmids, and this will need to be optimized and determined by the end user in all cases. 1. Liao J, Wu Z, Wang Y, et al. Enhanced efficiency of generating induced pluripotent stem (iPS) cells from human somatic cells by a combination of six transcription factors. Cell Res. 2008;18:600-603. 2. Dowey SN, Huang X, Chou BK, et al. Generation of integration-free human induced pluripotent stem cells from postnatal blood mononuclear cells by plasmid vector expression. Nat Protoc. 2012;7:2013-2021. 3. Meng X, Neises A, Su R-J, et al. Efficient Reprogramming of Human Cord Blood CD34+ Cells Into Induced Pluripotent Stem Cells With OCT4 and SOX2 Alone. Mol Ther. 2012;20:408-416. 4. Su R-J, Baylink DJ, Neises A, et al. Efficient Generation of Integration-Free iPS Cells from Human Adult Peripheral Blood Using BCL-XL Together with Yamanaka Factors. PLoS One. 2013;8:e64496. 5. Okita K, Yamakawa T, Matsumura Y, et al. An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells. Stem Cells. 2013;31:458-466.
|Can I amplify these episomal vectors?|
All commonly used bacterial strains (such as DH5alpha) can be used to propagate these plasmids, please also review the vector information/map for the corresponding resistance marker.
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