Column-Pure RNA Miniprep Kit

CAT.NOUNITPRICE
D51850 preparations
$285.00

Customize Column-Pure RNA Miniprep Kit
1 x Lysis Buffer   + $0.00
1 x Wash Buffer 1   + $0.00
1 x Wash Buffer 2 (Concentrate)   + $0.00
1 x DNase I (RNase-free)   + $0.00
1 x 10X DNase I Reaction Buffer   + $0.00
1 x Nuclease-Free H2O   + $0.00
1 x RNA Spin Columns, Collection Tubes   + $0.00

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Column-Pure RNA Miniprep Kit
Column-Pure RNA Miniprep Kit

In stock

$285.00

Summary
    Specifications


    Description

    abm’s Column-Pure RNA Miniprep Kit is a fast and efficient method for the isolation and purification of total RNA from mammalian cells, tissues, yeast and bacteria. The silica spin column technology allows for rapid recovery of high quality RNA that is ready for downstream applications such as RT-PCR, qPCR, cDNA libraries and Northern blotting.

    Advantages:
    •Rapid and simple 25 minute protocol

    •Extract high yield and purity RNA from mammalian cells, tissues, yeast and bacteria

    •On-column genomic DNA removal

    •High binding capacity of up to 200 µg RNA

    •Compatible with lower input number of cells (e.g. 100-1x107 HEK293T mammalian cells)

    •No phenol/chloroform, LiCl or alcohol precipitation required

    SKUD518
    Note

    Additional materials required (not supplied):

    Material

    Required for

    14.3 M β-mercaptoethanol (β-ME)

    All protocols

    95-100% Ethanol

    All protocols

    70% Ethanol

    All protocols

    Zymolyase or Lyticase and Reaction Buffer

    Yeast protocol

    Lysozyme

    Bacteria protocol

    Unit quantity50 preparations
    Documents


    Supporting Protocol
    MSDS
    QC

      Other

        FAQs


        Is it possible to use frozen blood sample with this kit?
        Frozen blood samples have not yet been tested with this kit, however this should not be a problem.
        What is the TE buffer used for?
        The TE buffer is optional and is included because some prefer to use TE for elution. TE buffer can be used instead of elution buffer if needed.
        References


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