Column-Pure RNA Miniprep Kit

CAT.NOUNITPRICE
D51850 preparations
$0.00

Specifications


Description

abm’s Column-Pure RNA Miniprep Kit is a fast and efficient method for the isolation and purification of total RNA from mammalian cells, tissues, yeast and bacteria. The silica spin column technology allows for rapid recovery of high quality RNA that is ready for downstream applications such as RT-PCR, qPCR, cDNA libraries and Northern blotting.

Advantages:
•Rapid and simple 25 minute protocol

•Extract high yield and purity RNA from mammalian cells, tissues, yeast and bacteria

•On-column genomic DNA removal

•High binding capacity of up to 200 µg RNA

•Compatible with lower input number of cells (e.g. 100-1x107 HEK293T mammalian cells)

•No phenol/chloroform, LiCl or alcohol precipitation required

SKUD518
Note

Additional materials required (not supplied):

Material

Required for

14.3 M β-mercaptoethanol (β-ME)

All protocols

95-100% Ethanol

All protocols

70% Ethanol

All protocols

Zymolyase or Lyticase and Reaction Buffer

Yeast protocol

Lysozyme

Bacteria protocol

Unit quantity50 preparations
Documents


Supporting Protocol
MSDS
QC

    Other

      FAQs


      Is it possible to use frozen blood sample with this kit?
      Frozen blood samples have not yet been tested with this kit, however this should not be a problem.
      What is the TE buffer used for?
      The TE buffer is optional and is included because some prefer to use TE for elution. TE buffer can be used instead of elution buffer if needed.
      References


      There are no references for this product yet!