Custom CRISPR Validation Service
Performed a CRISPR experiment, but unsure whether you have the right cell clone for your research? Looking for a third party to confirm your CRISPR results for publication? abm is proud to offer a variety of CRISPR validation options – from sgRNA efficiency screening, genomic cleavage assay, analysis by Sanger sequencing or NGS, to protein-level validation – to provide thorough validation of your CRISPR results.

"We are quite happy with the knockout cells that you made. This was a tricky knockout and hemizygous removal was much more prevalent than the double knockout, but you did it. In addition to your excellent genomic sequence characterization of both mutant LIF alleles, I have confirmed in our lab that the tumor cells have lost all mouse LIF production.""
Dr. Robert Jackman, Boston University, Mouse LIF CRISPR Knock Out C26 Tumor Cell Line Generation and Screening ServiceAdditional Resources:
Service Details
Service Name | Unit | CAT. NO. | UNIT PRICE |
---|---|---|---|
sgRNA Efficiency Screening Service
|
1 Service | C526 | $ 950.00 # |
Mismatch Cleavage Detection Service (Surveyor/T7E1 Assay)
|
1 Sample | C527 | $ 250.00 |
SpeedySeq Sequencing of Target Region
|
1 Sample | C528 | $ 385.00 |
SpeedySeq Sequencing of PCR Amplicon
|
1 Sample | C529 | $ 75.00 |
Validation Service by Western Blot (Up to 10 Clones) | 1 Service | C145 | $ 975.00 |
CRISPR-Cas9 Amplicon-Seq | 1 Sample | IA00100 | $ 600.00 |
Service Name | Unit | CAT. NO. | PRICE |
---|---|---|---|
gDNA Isolation from cell pellet | Per Cell Pellet | C530 | $ 50.00 |
Additional Info
Service Selection Guide
Service | Purpose | Materials to Submit | Deliverables |
---|---|---|---|
sgRNA Efficiency Screening Service | Validate sgRNA designs in vitro prior to performing CRISPR experiments |
|
Report with Cleavage assay gel image showing the cleavage efficiency of the different sgRNA designs |
Mismatch Cleavage Detection Service (Surveyor/T7E1 Assay) | Upon infection, determine if the polyclonal pool of cells contains edits |
|
Cleavage detection assay gel image |
SpeedySeq Sequencing of Target Region | Provides detailed sequence information of the CRISPR target region |
|
10 sequencing results/sample, service report showing alignment to reference sequence |
SpeedySeq Sequencing of PCR Amplicon | Used for preliminary screening to determine whether edits are present. Clean sequencing results can be seen for monoclonal samples. If messy sequencing results are seen at the expected sgRNA cleavage point, then the sample likely contains a mixture of edits/ sequences. |
|
1 sequencing result/sample, service report showing alignment to reference sequence |
Validation Service by Western Blot (Up to 10 Clones) | Protein-level validation |
|
Custom report on Western blot results |
CRISPR-Cas9 Amplicon-Seq | Can validate multiple samples at once and provides deeper sequencing coverage by NGS |
|
FASTQ data |
Off-Target Analysis by Whole Genome Sequencing | Determines off-targets |
|
FASTQ data |
- Primer design and synthesis (1 set of primers)
- sgRNA template synthesis
- In vitro transcription of sgRNAs
- Amplification of DNA target(s) from gDNA
- In vitro cleavage assay with Cas Nuclease Protein (please choose one from the available list: saCas9, spCas9, fnCpf1, asCpf1)
Lead time: 2-4 weeks
Mismatch Cleavage Detection Service (Surveyor/T7E1 Assay):
- Primer design and synthesis (up to 2 sets of primers tested)
- Amplify region of interest from gDNA
- Mismatch cleavage detection assay, 1 reaction/sample
Lead time: 3-5 weeks
SpeedySeq Sequencing of Target Region
- Primer design and synthesis (up to 2 sets of primers tested)
- Amplification of DNA target from gDNA
- Subcloning
- Culture and isolate 10 clones per sample
- Sanger sequencing of clones (target region sequenced is up to 700bp)
- Alignment to reference sequence
Lead time: 3-5 weeks
SpeedySeq Sequencing of PCR Amplicon
- Primer design and synthesis (up to 2 sets of primers tested)
- Amplification of DNA target from gDNA
- Sanger sequencing of PCR product
- Alignment to reference sequence
Deliverables: 1 sequencing result/sample, service report showing chromatogram and alignment to reference sequence
Lead time: 2-4 weeks
Protein CRISPR Validation Service
- Western blot will be performed on wild-type sample, CRISPR-edited samples, and the positive control using pre-validated antibody.
Lead time: 2-4 weeks
*All lead times specified are estimates and based on receipt of gDNA or protein lysate samples. If a gDNA isolation service is required, 1 additional week will be added.
Sample Submission Guideline
Sample format | Details |
---|---|
gDNA |
|
Cell pellet |
|
Disclaimer and Policies
Our goal is always to deliver high-quality validation data to aid you in your research and downstream application. However, CRISPR validation service results are dependent on the sample quality submitted by customers and as such, abm is not in a position to guarantee the final results obtained and full service charges will apply for the services performed. By placing an order with abm, customer agrees to this term.
All services are for research use only.