Custom miR-Tuners

Conventional mammalian expression systems rely on the usage of strong viral promoters (i.e. CMV and SV40) which in turn over-express the gene of interest (GOI). However, over-expression of a foreign gene can lead to detrimental effects in the cell and importantly, fails to replicate normal physiological gene expression levels. To address this issue, miR-Tuners can be used to precisely fine-tune transgene expression levels in a predictable manner.

miR-Tuners are short, synthetic miRNA response element sequences which reside in the 3’ UTR of the GOI. These sequences are responsive to a native miRNA species which is widely expressed in many human and mouse cells. Using the miR-Tuner Low, Med or Hi will allow for precise low, medium or high expression levels of the GOI, respectively.

abm offers a variety of miR-Tuner products which can precisely and predictably control transgene expression levels in mammalian cells. These products include ready-to-clone miR-Tuner MCS (multiple cloning site) Lentiviral Vectors and custom cloning services of your GOI into a set of miR-Tuner Vectors and/or Lentiviral packaging.

Applications
Fine-tuning transgene expression levels in mammalian cells for:

  • Engineered genetic circuits and cell lines
  • Studying physiological and biochemical changes


Looking to clone your own miR-Tuner vectors? Check out our individual products here



Workflow

"Always amazed at the reasonable prices and breadth of selection for genes of interest available on your site. Thanks very much!"

Brian DeBosch, Washington University in St. Louis

Additional Resources:

Service Details

ServiceUnitCat. No.Price
1. Choose Insert Format
a. ORF Not a Deliverable N/A $45.00
b. Gene Synthesis Per bp C098 From $0.1/bp
2. Choose Product Format
a. Vector Options:
miR-Tuners Set of 3 custom cloned vectors (puromycin) 3 x 10 µg LV106 $895.00
miR-Tuners Set of 3 custom cloned vectors (NGFR) 3 x 10 µg LV107 $995.00
b. Lentivirus Options:
miR-Tuners Set of 3 lentivirus (puromycin), 107 IU/ml 2 x 200 µl each virus LV108 $1850.00
miR-Tuners Set of 3 lentivirus (NGFR), 107 IU/ml 2 x 200 µl each virus LV109 $1950.00
Controls:
ProductUnitCAT. NO.Price
miR-Tuners Set of 3 Control vectors
LV104-Low
LV104-Med
LV104-Hi
3 x 10 µg LV104 $350.00
miR-Tuners Set of 3 Control Lentiviruses, 107 IU/ml
LV105-Low
LV105-Med
LV105-Hi
3 x 200 µl LV105 $850.00

Additional Info

  • Workflow

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