DNA Marker

CAT.NOUNITPRICE
$40.00

Specifications


Description

abm's offers a wide range of DNA Markers, ranging from 50bp to 25 kb in size. Each DNA marker has certain bands with increased intensity to serve as quick reference points. In addition, approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.

We offer five different DNA Markers 

• 17 bands of DNA Ranging from 50bp-1.5kb (Cat. No. G016, 100bp Opti-DNA Marker)
• 12 bands of DNA Ranging from 100bp-3kb (Cat. No. G193, 100bp Plus Opti-DNA Marker)
• 19 bands of DNA Ranging from 100bp-10kb (Cat. No. G106, 1kb Opti-DNA Marker)
• 14 bands of DNA Ranging from 250bp-25kb (Cat. No. G248, 1kb Plus Opti-DNA Marker)
• 8 bands of DNA Ranging from 100bp-5kb (Cat. No. G249, 2kb Opti-DNA Marker)

SKUDNA Marker
Applications

For approximating DNA size and amount on agarose gels.

Suggested LoadMix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.
Shipping Conditions

Shipped on blue ice packs.

Storage Condition

Store @ 4°C for up to 3 months or -20°C for up to 24 months.

FAQs


Is it ready for use?
Yes, it is ready to use. Just load suggested volume (5ul) to the well.
What is the smallest size agarose gel can separate?
That is around 300bp for 1% agarose and 100bp for 2.5% agarose. With anything smaller than the dye, there may be separation but it will be difficult to tell when the fragment will run off the gel.
Are the Opti-DNA markers compatible with radiolabelling?
Our lab has not tested the Opti-DNA markers for this particular application, but we believe it shouldn't be a problem. The Opti-DNA markers should be compatible for radio-labelling.
References


1
  • Gnanandarajah, JS et al. "Identification by mass spectrometry and immune response analysis of guinea pig cytomegalovirus (GPCMV) pentameric complex proteins GP129, 131 and 133" Viruses 6 (2):727-751 (2014). DOI: 10.3390/v6020727. PubMed: 24531333. Application: PCR Products size reference.