DNA Marker

CAT.NO	UNIT	PRICE
		$40.00

Specifications

Print Version

Description	abm's offers a wide range of DNA Markers, ranging from 50bp to 25 kb in size. Each DNA marker has certain bands with increased intensity to serve as quick reference points. In addition, approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size. We offer five different DNA Markers • 17 bands of DNA Ranging from 50bp-1.5kb (Cat. No. G016, 100bp Opti-DNA Marker) • 12 bands of DNA Ranging from 100bp-3kb (Cat. No. G193, 100bp Plus Opti-DNA Marker) • 19 bands of DNA Ranging from 100bp-10kb (Cat. No. G106, 1kb Opti-DNA Marker) • 14 bands of DNA Ranging from 250bp-25kb (Cat. No. G248, 1kb Plus Opti-DNA Marker) • 8 bands of DNA Ranging from 100bp-5kb (Cat. No. G249, 2kb Opti-DNA Marker)
SKU	DNA Marker
Applications	For approximating DNA size and amount on agarose gels.
Suggested Load	Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.
Shipping Conditions	Shipped on blue ice packs.
Storage Condition	Store @ 4°C for up to 3 months or -20°C for up to 24 months.
Description	PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 17 bands, ranging from 50 bp – 1.5 kb, for molecular weight standards in agarose gel electrophoresis. The 200 and 500 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
SKU	G016
Applications	For approximating DNA size and amount on agarose gels.
Bands	17
DNA Range	50bp-1.5kb
Note	Concentration of this marker is 112 μg / ml
Suggested Load	Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.
Shipping Conditions	Shipped on blue ice packs.
Storage Condition	Store @ 4°C for up to 3 months or -20°C for up to 24 months.
Unit quantity	500 μl/100 loads
Description	PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 19 bands, ranging from 100 pb – 10 kb, for molecular weight standards in agarose gel electrophoresis. The 500, 1,500, and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
SKU	G106
Applications	For approximating DNA size and amount on agarose gels.
Bands	19
DNA Range	100bp-10kb
Note	Concentration of this marker is 172 μg / ml
Suggested Load	Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.
Shipping Conditions	Shipped on blue ice packs.
Storage Condition	Store @ 4°C for up to 3 months or -20°C for up to 24 months.
Unit quantity	500 μl/100 loads
Description	PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 14 bands, ranging from 250 pb – 25 kb, for molecular weight standards in agarose gel electrophoresis. The 1,000 and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size. Total DNA concentration for all bands combined is 103.2ng/μl.
SKU	G248
Applications	For approximating DNA size and amount on agarose gels.
Bands	14
DNA Range	250bp-25kb
Note	Concentration of this marker is 104 μg / ml
Suggested Load	Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.
Shipping Conditions	Shipped on blue ice packs.
Storage Condition	Store @ 4°C for up to 3 months or -20°C for up to 24 months.
Unit quantity	500 μl/100 loads
Description	PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 12 bands, ranging from 100 pb – 3 kb, for molecular weight standards in agarose gel electrophoresis. The 500 and 1,500 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
SKU	G193
Applications	For approximating DNA size and amount on agarose gels.
Bands	12
DNA Range	100bp-3kb
Note	Concentration of this marker is 108 μg / ml
Suggested Load	Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.
Shipping Conditions	Shipped on blue ice packs.
Storage Condition	Store @ 4°C for up to 3 months or -20°C for up to 24 months.
Unit quantity	500 μl/100 loads
Description	PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 8 bands, ranging from 100 pb – 5 kb, for molecular weight standards in agarose gel electrophoresis. The 750 base pair band has increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
SKU	G249
Applications	For approximating DNA size and amount on agarose gels.
Bands	8
DNA Range	100bp-5kb
Suggested Load	Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.
Shipping Conditions	Shipped on blue ice packs.
Storage Condition	Store @ 4°C for up to 3 months or -20°C for up to 24 months.
Unit quantity	500 μl/100 loads

Documents

Supporting Protocol

DNA Marker Guide

MSDS

Other

FAQs

	Is it ready for use?
Yes, it is ready to use. Just load suggested volume (5ul) to the well.

	What is the smallest size agarose gel can separate?
That is around 300bp for 1% agarose and 100bp for 2.5% agarose. With anything smaller than the dye, there may be separation but it will be difficult to tell when the fragment will run off the gel.

	Are the Opti-DNA markers compatible with radiolabelling?
Our lab has not tested the Opti-DNA markers for this particular application, but we believe it shouldn't be a problem. The Opti-DNA markers should be compatible for radio-labelling.

References

Gnanandarajah, JS et al. "Identification by mass spectrometry and immune response analysis of guinea pig cytomegalovirus (GPCMV) pentameric complex proteins GP129, 131 and 133" Viruses 6 (2):727-751 (2014). DOI: 10.3390/v6020727. PubMed: 24531333. Application: PCR Products size reference.