DNA Polymerase I Large (Klenow) Fragment
|E013||500 U (100 μl)|
|Description||DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I (abm Cat. No. G469). The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5’→3’ exonuclease activity. With its inherent 3’→5’ exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini.|
|Unit quantity||500 U (100 μl)|
|Format||Enzyme supplied with 10X Reaction Buffer|
Store all components at -20°C.
|Storage Buffer||25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.|
One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
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