DNA Polymerase I Large (Klenow) Fragment

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E013500 U (100 μl)
$45.00

Customize DNA Polymerase I Large (Klenow) Fragment
1 x Klenow DNA Polymerase   + $0.00
1 x 10X Klenow Buffer   + $0.00

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DNA Polymerase I Large (Klenow) Fragment
DNA Polymerase I Large (Klenow) Fragment

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$45.00

Summary
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    DescriptionDNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I (abm Cat. No. G469). The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5’→3’ exonuclease activity. With its inherent 3’→5’ exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini.
    SKUE013
    Unit quantity500 U (100 μl)
    Applications
    • DNA blunting by filling-in 5'-overhangs with unlabeled or labeled dNTPs
    • cDNA second-strand synthesis
    • Generate single-stranded DNA probes using random primers
    • Site-directed DNA mutagenesis using synthetic oligonucleotides
    • Dideoxy DNA sequencing of single- or double-stranded DNA templates
    • 3’→5’ exonuclease activity can blunt a 3’-overhang
    Concentration5 U/μl
    FormatEnzyme supplied with 10X Reaction Buffer
    Storage Condition

    Store all components at -20°C.

    Storage Buffer25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
    Note

    One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
    This product is distributed for laboratory research only. Caution: Not for diagnostic use.

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