DNAfectin™ Plus Transfection Reagent
Cat. No.
G2500
Unit
1.0 ml
Price
$195.00
Specifications
| Description |
High Transfection Efficiency!abm's DNAfectin™ Plus Transfection Reagent is a unique formulation of polycations and liposomes, which guarantees high transfection efficiency in a wide variety of cell types including primary cells. Product Features:
|
| Cat. No. | G2500 |
| Name | DNAfectin™ Plus Transfection Reagent |
| Sterility |
Store at 4ºC. Do not freeze. |
| Application |
Transfection of DNA into cultured eukaryotic cells. |
| Storage Condition |
Store at 4ºC. Do not freeze. |
STR
There are no STR for this product yet!
FAQs
| How much transfection reagent and DNA do I need per well in a six well plate? | |
|
5ug DNA and 10ul of transfection reagent will be fine. If toxicity is a problem, use 3~4 ug DNA.
|
| Does freeze-thaw affect the performance of the transfection reagent? | |
|
Freeze-thaw does damage the transfection reagent slightly, at an approximate 5% loss.
If possible, test the agent with a GFP control first.
|
| Is this polymer or metal based? | |
|
This item is polymer based.
|
| I used this transfection reagent but I see toxicity, what are some tips to resolve this? | |
|
Try reducing the amount of DNA plasmids and Transfection Reagent added by half.
|
References
- Huang, J et al. "Long non-coding RNA UCA1 promotes breast tumor growth by suppression of p27 (Kip1)" Cell Death Dis 5:e1008 (2014). DOI: 10.1038/cddis.2013.541. PubMed: 24457952.
- Singh, R et al. "Exosome-mediated transfer of miR-10b promotes cell invasion in breast cancer" Molecular Cancer 13(1):256 (2014). DOI: 10.1186/1476-4598-13-256. PubMed: 25428807.
- Ott, C.A. et al. "Cell Lysate-Based AlphaLISA Deubiquitinase Assay Platform for Identification of Small Molecule Inhibitors" ACS Chem. Biol. Web: (2017). DOI: 10.1021/acschembio.7b00543. PubMed: 28836754.
- Ji, Y., Li, L., Ma, Y.-X., Li, W.-T., Li, L., Zhu, H.-Z., … Zhou, J.-R. "Quercetin inhibits growth of hepatocellular carcinoma by apoptosis induction in part via autophagy stimulation in mice" The Journal of Nutritional Biochemistry 69:108–119 (2019). DOI: 10.1016/j.jnutbio.2019.03.018.
- Li, K., Liu, Y., Xu, Z., Zhang, Y., Luo, D., Gao, Y., ... & Qi, X. "Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion" PLoS pathogens 15(9): (2019).
- Peng, W. X., Koirala, P., Zhang, W., Ni, C., Wang, Z., Yang, L., & Mo, Y. Y. "LncRNA RMST enhances DNMT3 expression through interaction with HuR" Molecular Therapy. : (2019).
- Zhang, S., Yang, Y., Huang, S., Deng, C., Zhou, S., Yang, J., … Zou, X. "SIRT1 inhibits gastric cancer proliferation and metastasis via STAT3/MMP‐13 signaling" Journal of Cellular Physiology 234(9):15395–15406 (2019). DOI: 10.1002/jcp.28186.
Controls and Related Product:
Other Cell Lines
Select Species:
African Green Monkey (C. aethiops)
(0)
Bat
(0)
Bee
(0)
Bottlenose Dolphin (Tursiops)
(0)
Cabbage Looper (T. ni)
(0)
Cat (Feline)
(0)
Cattle
(0)
Chicken (Galline)
(0)
Chinese Hamster (C. griseus)
(0)
Cow (Bovine)
(0)
Dog (Canine)
(0)
Duck (Anas)
(0)
Duck (Anatidae)
(0)
E. coli
(0)
European Sea Bass
(0)
European Sea Bass (D. labrax)
(0)
Fall Armyworm (S. frugiperda)
(0)
Fish
(0)
Fly (D. melanogaster)
(0)
Frog (Xenopus)
(0)
Fruit Fly (D. melanogaster)
(0)
Gilthead Sea Bream (S. aurata)
(0)
Goat (Capra)
(0)
Golden Hamster (M. auratus)
(0)
Goose (Anatidae)
(0)
Green Monkey (C. aethiops)
(0)
Green Monkey (C. sabaeus)
(0)
Hamster (M. auratus)
(0)
Horse (Equine)
(0)
Human (H. sapiens)
(0)
Insect
(0)
Killifish (F. heteroclitus)
(0)
Luciola italica
(0)
Marmoset (Primate)
(0)
Mink
(0)
Monkey (Primate)
(0)
Moth (S. frugiperda)
(0)
Mouse (M. musculus)
(0)
Other
(0)
Photinus pyralis
(0)
Pig (Porcine)
(0)
Quail (Coturnix)
(0)
Rabbit (Leporidae)
(0)
Rabbit (Leporine)
(0)
Rainbow Trout (O. mykiss)
(0)
Rat (R. norvegicus)
(0)
Rat (Rattus)
(0)
Renilla reniformis
(0)
Roundworm (C. elegans)
(0)
Rous Sarcoma Virus (RSV)
(0)
SARS-CoV-2
(0)
Sheep (Ovine)
(0)
Sheep (Ovis)
(0)
Turtle
(0)
Universal
(0)
Zebrafish (D. rerio)
(0)
Select Tissue:
0
(0)
Achilles Tendon
(0)
Adipose
(0)
Adrenal Gland
(0)
Adrenal Glands
(0)
Airway/Lung
(0)
Amniotic Sac
(0)
Antimesometrial Decidua
(0)
antler
(0)
Ascites
(0)
Auditory
(0)
Bee Cell
(0)
Bile Duct
(0)
Bladder
(0)
Blood
(0)
Blood Vessel
(0)
Bone
(0)
Bone Marrow
(0)
Brain
(0)
Breast
(0)
Caput Epididymis
(0)
Cartilage
(0)
Cerebrospinal Fluid
(0)
Cervix
(0)
Colon
(0)
Connective Tissue
(0)
Cord Blood
(0)
Digestive
(0)
Digestive System
(0)
Embryo
(0)
embryonic
(0)
Endometria
(0)
Endometrium
(0)
Esophagus
(0)
Eye
(0)
Fibroblast
(0)
Gingiva
(0)
Hair
(0)
Hair Follicle
(0)
Heart
(0)
HeLa
(0)
Hepatocellular Carcinoma
(0)
Hippocampus
(0)
Intestinal
(0)
Intestine
(0)
Kidney
(0)
Kidney, Cortex/Proximal tubule
(0)
Labial Salivary Gland
(0)
Larynx
(0)
Liver
(0)
Lung
(0)
Lung Carcinoma
(0)
Lung, bronchus
(0)
Lymph node
(0)
Lymphatic System
(0)
lymphoblast
(0)
Malignant melanoma
(0)
Mammary
(0)
Mesencephalic Tissue
(0)
Mesentery
(0)
Microglia
(0)
Muscle
(0)
Myoblast
(0)
Nasal Mucosa
(0)
Nerve
(0)
Nervous System
(0)
Oesophageal
(0)
Oesophagus
(0)
Oral
(0)
Organ of Corti
(0)
Other
(0)
Ovary
(0)
Pancreas
(0)
Pancreatic
(0)
Parathyroid
(0)
Peripheral Blood
(0)
Peripheral Blood Mononuclear Cells
(0)
Peritoneal
(0)
Peritoneum
(0)
Pituitary
(0)
Pituitary Gland
(0)
Pituitary Tumor
(0)
Placenta
(0)
promyelocytic leukemia
(0)
Prostate
(0)
Prostate carcinoma
(0)
Renal
(0)
Reproductive
(0)
Reproductive System
(0)
Retina
(0)
Salivary Gland
(0)
Sciatic Nerve
(0)
Skeletal
(0)
Skeletal Muscle
(0)
Skin
(0)
Skin ; Keratinocytes
(0)
Small intestine
(0)
Soft Tissue
(0)
Spleen
(0)
Stem Cell
(0)
Stomach
(0)
Straitum
(0)
Sweat Gland
(0)
Testis
(0)
Thymic
(0)
Thymus
(0)
Thyroid
(0)
Tongue
(0)
Tonsil
(0)
Trachea
(0)
Tumor
(0)
Unknown
(0)
Uterus
(0)
Uterus; endometrium
(0)
Vascular Endothelium
(0)
Ventral Mesencephalon
(0)
Vertebral Disc
(0)