DNAfectin™ Plus Transfection Reagent

CAT.NOUNITPRICE
G25001.0 ml
$195.00

Specifications


Description

Transfection made easy! DNAfectin™ Plus Transfection Reagent offers a simple protocol that does not require the removal of serum or culture medium, resulting in less variability and low risk of contamination. This powerful nanoparticle-based formulation enables the achievement of higher transfection efficiencies and lower toxicity compared to other types of reagents.

SKUG2500
Applications

Transfection of DNA into cultured eukaryotic cells.

Transfection Reagents TypeDNA Molecule
Unit quantity1.0 ml
Note

Please check our Transfection Cell Database for tested cell lines

DNAfectin™ Transfection Reagent is a special and unique formulation of polycations, offering a high transfection efficiency with low cytotoxicity for many cell types, including primary cells. In contrast, DNAfectin™ Plus is a nanoparticle-based transfection reagent. This innovative technology allows DNAfectin™ Plus to achieve superior and reproducible transgene expression levels with no cytotoxic effects. DNAfectin™ Plus also offers a simple protocol that is serum-compatible.

Transfection Database
Our Transfection Cell Database contains data from transduction results from cell lines tested by in house scientists at ABM. This is a continuing effort and more cell lines will be added to the database once the data is available. Please search our database below for specific details. Email us your transfection results for cell lines not listed below at [email protected] to receive a FREE gift.

Cell LineCell Line Species/TissueNucleic AcidReagentTransfection TypeTransfection Efficiency(%)
293T Human Embryonic Kidney DNA DNAfectinTM 2100 Transfection Reagent Transient 100%
Jurkat Human T Lymphoma DNA DNAfectinTM 2100 Transfection Reagent Transient 25%
SHEP Human Neuroblastoma DNA DNAfectinTM 2100 Transfection Reagent Transient 90%
BOSC23 Human Kidney DNA DNAfectinTM 2100 Transfection Reagent Transient 90%
HT1080 Human Fibrosarcoma DNA DNAfectinTM 2100 Transfection Reagent Transient 80%
C33A Human Cervical Carcinoma DNA DNAfectinTM 2100 Transfection Reagent Transient 90%
MRC5 Human Primary Fibroblast DNA DNAfectinTM 2100 Transfection Reagent Transient 80%
MDK-Telo Dog Kidney Cells+Telo DNA DNAfectinTM 2100 Transfection Reagent Transient 80%
B16 Murine Melanoma DNA DNAfectinTM 2100 Transfection Reagent Transient 80%
CB3 Mouse Erytholeukemia DNA DNAfectinTM 2100 Transfection Reagent Transient 25%
HuVEC Human Ubilical Vein DNA DNAfectinTM 2100 Transfection Reagent Transient 20%
HepG2 Human Liver Carcinoma DNA DNAfectinTM 2100 Transfection Reagent Transient 40%
MMRU Human Melanoma DNA DNAfectinTM 2100 Transfection Reagent Transient 75%
MMAN Human Melanoma DNA DNAfectinTM 2100 Transfection Reagent Transient 80%
MDA-MB-231 Human Breast Carcinoma DNA DNAfectinTM 2100 Transfection Reagent Transient 85%
HeLa Human Cervical Carcinoma DNA DNAfectinTM 2100 Transfection Reagent Transient 80%-90%
A2780 Human Ovarian Carcinoma DNA DNAfectinTM 2100 Transfection Reagent Transient 70%-75%
L6 Rat Myoblast DNA DNAfectinTM 2100 Transfection Reagent Transient 60%-70%
Rabbit-telo Telomerase-Rabbit Cells DNA DNAfectinTM 2100 Transfection Reagent Transient 50%
USO2 (T-USO2) Human Osteosarcoma (tet-inducible) DNA DNAfectinTM 2100 Transfection Reagent Transient 80%-90%
Storage Condition

Store at 4ºC. Do not freeze.

Documents


Supporting Protocol
MSDS

    QC
    Other

    FAQs


    How much transfection reagent and DNA do I need per well in a six well plate?
    5ug DNA and 10ul of transfection reagent will be fine. If toxicity is a problem, use 3~4 ug DNA.
    Does freeze-thaw affect the performance of the transfection reagent?
    Freeze-thaw does damage the transfection reagent slightly, at an approximate 5% loss. If possible, test the agent with a GFP control first.
    Is this polymer or metal based?
    This item is polymer based.
    References


    7
    • Huang, J et al. "Long non-coding RNA UCA1 promotes breast tumor growth by suppression of p27 (Kip1)" Cell Death Dis 5:e1008 (2014). DOI: 10.1038/cddis.2013.541. PubMed: 24457952.
    • Singh, R et al. "Exosome-mediated transfer of miR-10b promotes cell invasion in breast cancer" Molecular Cancer 13(1):256 (2014). DOI: 10.1186/1476-4598-13-256. PubMed: 25428807.
    • Ott, C.A. et al. "Cell Lysate-Based AlphaLISA Deubiquitinase Assay Platform for Identification of Small Molecule Inhibitors" ACS Chem. Biol. Web: (2017). DOI: 10.1021/acschembio.7b00543. PubMed: 28836754.
    • Ji, Y., Li, L., Ma, Y.-X., Li, W.-T., Li, L., Zhu, H.-Z., … Zhou, J.-R. "Quercetin inhibits growth of hepatocellular carcinoma by apoptosis induction in part via autophagy stimulation in mice" The Journal of Nutritional Biochemistry 69:108–119 (2019). DOI: 10.1016/j.jnutbio.2019.03.018.
    • Li, K., Liu, Y., Xu, Z., Zhang, Y., Luo, D., Gao, Y., ... & Qi, X. "Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion" PLoS pathogens 15(9): (2019).
    • Peng, W. X., Koirala, P., Zhang, W., Ni, C., Wang, Z., Yang, L., & Mo, Y. Y. "LncRNA RMST enhances DNMT3 expression through interaction with HuR" Molecular Therapy. : (2019).
    • Zhang, S., Yang, Y., Huang, S., Deng, C., Zhou, S., Yang, J., … Zou, X. "SIRT1 inhibits gastric cancer proliferation and metastasis via STAT3/MMP‐13 signaling" Journal of Cellular Physiology 234(9):15395–15406 (2019). DOI: 10.1002/jcp.28186.