DNAfectin™ Plus Transfection Reagent

CAT.NOUNITPRICE
G25001.0 ml
$195.00

Specifications


Description

High Transfection Efficiency!

abm's DNAfectin™ Plus Transfection Reagent is a unique formulation of polycations and liposomes, which guarantees high transfection efficiency in a wide variety of cell types including primary cells.

Product Features:

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2X the points for PCR Enzymes, Buffers, Solutions, and more!

SKUG2500
Applications

Transfection of DNA into cultured eukaryotic cells.

Transfection Reagents TypeDNA Molecule
Unit quantity1.0 ml
Storage Condition

Store at 4ºC. Do not freeze.

Documents


Supporting Protocol
MSDS

    QC
    Other

    FAQs


    How much transfection reagent and DNA do I need per well in a six well plate?
    5ug DNA and 10ul of transfection reagent will be fine. If toxicity is a problem, use 3~4 ug DNA.
    Does freeze-thaw affect the performance of the transfection reagent?
    Freeze-thaw does damage the transfection reagent slightly, at an approximate 5% loss. If possible, test the agent with a GFP control first.
    Is this polymer or metal based?
    This item is polymer based.
    I used this transfection reagent but I see toxicity, what are some tips to resolve this?
    Try reducing the amount of DNA plasmids and Transfection Reagent added by half.
    References


    7
    • Huang, J et al. "Long non-coding RNA UCA1 promotes breast tumor growth by suppression of p27 (Kip1)" Cell Death Dis 5:e1008 (2014). DOI: 10.1038/cddis.2013.541. PubMed: 24457952.
    • Singh, R et al. "Exosome-mediated transfer of miR-10b promotes cell invasion in breast cancer" Molecular Cancer 13(1):256 (2014). DOI: 10.1186/1476-4598-13-256. PubMed: 25428807.
    • Ott, C.A. et al. "Cell Lysate-Based AlphaLISA Deubiquitinase Assay Platform for Identification of Small Molecule Inhibitors" ACS Chem. Biol. Web: (2017). DOI: 10.1021/acschembio.7b00543. PubMed: 28836754.
    • Ji, Y., Li, L., Ma, Y.-X., Li, W.-T., Li, L., Zhu, H.-Z., … Zhou, J.-R. "Quercetin inhibits growth of hepatocellular carcinoma by apoptosis induction in part via autophagy stimulation in mice" The Journal of Nutritional Biochemistry 69:108–119 (2019). DOI: 10.1016/j.jnutbio.2019.03.018.
    • Li, K., Liu, Y., Xu, Z., Zhang, Y., Luo, D., Gao, Y., ... & Qi, X. "Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion" PLoS pathogens 15(9): (2019).
    • Peng, W. X., Koirala, P., Zhang, W., Ni, C., Wang, Z., Yang, L., & Mo, Y. Y. "LncRNA RMST enhances DNMT3 expression through interaction with HuR" Molecular Therapy. : (2019).
    • Zhang, S., Yang, Y., Huang, S., Deng, C., Zhou, S., Yang, J., … Zou, X. "SIRT1 inhibits gastric cancer proliferation and metastasis via STAT3/MMP‐13 signaling" Journal of Cellular Physiology 234(9):15395–15406 (2019). DOI: 10.1002/jcp.28186.