DpnI
| CAT.NO | UNIT | PRICE |
|---|---|---|
| E026 | 2000 U (100 μl) | $65.00 |
| Description | The DpnI restriction enzyme digests DNA at GmeA↓TC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves hemi-methylated dam sites 60 X more slowly than fully methylated. |
|---|---|
| SKU | E026 |
| Unit quantity | 2000 U (100 μl) |
| Applications |
|
| Concentration | 20 U/μl |
| Format | Enzyme supplied with 10X Reaction Buffer |
| Storage Condition | -20°C |
| Storage Buffer | 10 mM Tris-HCl (pH 7.5), 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 µg/ml BSA, and 50% (v/v) Glycerol. |
| Note | One unit is defined as the amount of Dpn1 required to digest 1 µg of dam methylated pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 µl. |
Supporting Protocol
MSDS
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