DpnI

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E0262000 U (100 μl)
$65.00

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DpnI
DpnI

In stock

$65.00

Summary
    Specifications


    Description

    The DpnI restriction enzyme digests DNA at GmeA↓TC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves hemi-methylated dam sites 60 X more slowly than fully methylated.

    SKUE026
    Unit quantity2000 U (100 μl)
    Applications
    • Molecular cloning
    • Site directed mutagenesis
    • Restriction site mapping
    • Genotyping
    • Southern Blot
    • SNP
    • Restriction fragment length polymorphism (RFLP)
    Concentration20 U/μl
    FormatEnzyme supplied with 10X Reaction Buffer
    Storage Condition

    -20°C

    Storage Buffer10 mM Tris-HCl (pH 7.5), 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 µg/ml BSA, and 50% (v/v) Glycerol.
    Note

    One unit is defined as the amount of Dpn1 required to digest 1 µg of dam methylated pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
    This product is distributed for laboratory research only. Caution: Not for diagnostic use.

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