DpnI
Cat. No. | E026 |
Name | DpnI |
Unit | 2000 U (100 μl) |
Category | Molecular Biology Enzymes and Kits |
Description |
The DpnI restriction enzyme digests DNA at GmeA↓TC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam+ E. coli strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves hemi-methylated dam sites 60X more slowly than fully methylated dam sites. |
Reaction Definition | One unit is defined as the amount of Dpn1 required to digest 1 µg of dam methylated pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 µl. |
Note |
Heat inactivation 65°C for 20 minutes. |
Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E026 |
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