|E026||2000 U (100 μl)|
The DpnI restriction enzyme digests DNA at GmeA↓TC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves hemi-methylated dam sites 60 X more slowly than fully methylated.
|Unit quantity||2000 U (100 μl)|
|Format||Enzyme supplied with 10X Reaction Buffer|
|Storage Buffer||10 mM Tris-HCl (pH 7.5), 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 µg/ml BSA, and 50% (v/v) Glycerol.|
One unit is defined as the amount of Dpn1 required to digest 1 µg of dam methylated pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
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