DpnI

Cat. No.
E026
Unit
2000 U (100 μl)
Price
$78.50
Cat. No. E026
Name DpnI
Unit 2000 U (100 μl)
Category Molecular Biology Enzymes and Kits
Description

The DpnI restriction enzyme digests DNA at GmeA↓TC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam+ E. coli strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves hemi-methylated dam sites 60X more slowly than fully methylated dam sites.

Reaction Definition One unit is defined as the amount of Dpn1 required to digest 1 µg of dam methylated pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Note

Heat inactivation

65°C for 20 minutes.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E026
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