EcoRI

CAT.NOUNITPRICE
E04820000 U (1 ml)
$45.00

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EcoRI
EcoRI

In stock

$45.00

Summary
    Specifications


    SKUE048
    Unit quantity20000 U (1 ml)
    Applications

    Restriction enzyme digestion of double-stranded DNA

    Components

    EcoRI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)

    Concentration20000 U/ml
    Expression SystemAn E. coli strain that carries the modified EcoRI gene from E. coli RY13. The EcoRI enzyme recognizes and cuts the sequence G^AATTC.
    Inactivation Protocol65°C for 20 min
    Reaction Definition1X Universal Restriction Enzyme Reaction Buffer (Cat. No. E204). Incubate at 37°C
    Reaction Buffer

    Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%
    Click here to view the buffer compatibility chart for restriction enzymes.

    Storage Condition

    -20°C

    Storage Buffer20 mM Tris-HCl (pH 7.5), 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50% (v/v) Glycerol
    Note

    Methylation Sensitivity
    dam methylation: Not Sensitive
    dcm methylation: Not Sensitive
    CpG Methylation: Blocked by Some Combinations of Overlapping

    Documents


    Supporting Protocol
    MSDS

      QC
      Other

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