Exonuclease I, E. coli

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E0152000 U (100 μl)
$15.00

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Exonuclease I, E. coli
Exonuclease I, E. coli

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$15.00

Summary
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    DescriptionExonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3’→5’ direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the 3’-terminus is phosphorylated.
    SKUE015
    Unit quantity2000 U (100 μl)
    Applications
    • Removal of single-stranded primer oligonucleotides from: o PCR mixtures for applications involving sequencing or labelling o from nucleic acid mixtures
    • Assaying for the presence of single-stranded DNA with a 3'-hydroxyl terminus
    • Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
    Concentration20 U/μl
    FormatEnzyme supplied with 10X Reaction Buffer
    Storage Condition

    Store all components at -20°C.

    Storage Buffer20 mM HEPES (pH 7.1), 150 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50% (v/v) Glycerol.
    Note

    One unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer.
    This product is distributed for laboratory research only. Caution: Not for diagnostic use.

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