FGFR1 Expressing Stable Ba/F3 (ZNF) Cell Line

CAT.NOUNITPRICE
T61451x106 cells / 1.0 ml
$0.00

Specifications


Description

Fusing a kinase domain to a homodimerization domain such as the ZNF domain can result in activated kinase. The FGFR1 Expressing Stable Ba/F3 (ZNF) Cell Line expresses activated human FGFR1 tyrosine kinase (ZNF-FGFR1 in MSCV vector). Ba/F3 cells expressing activated tyrosine kinase become completely dependent on the kinase activity for survival and as a result, the cells can be propagated even in the absence of IL-3.

The FGF family of receptor tyrosine kinases consist of proteins which bind to fibroblast growth factors. Family members are involved in numerous developmental processes, which include limb development and brain patterning. Recent research has shown them to be important in metabolism and phosphate levels in the body. Mutations in FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving FRFR1 are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome.

SKUT6145
SpeciesMouse (M. musculus)
Tissue/Organ/Organ SystemBone Marrow
Growth PropertiesSuspension
Cell MorphologyRound
Seeding Density50,000 - 100,000 cells/ml
Population Doubling Time20 - 30 hours
Applications

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetKinases
Cell TypeDrug Discovery Cell Lines
Propagation Requirements

The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
QC

1) FACS

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

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