FLAG-HnRNP-L Stable Expressing HEK293 Cell Line

T62501x106 cells / 1.0 ml


DescriptionActively transcribed genes are mediated by KMT3a in humans. Within that protein complex is a eukaryotic specific subunit heterogeneous nuclear ribonucleoprotein L (HnRNP-L). HnRNP-L is a member of a large family of RRM domain containing RNA-binding proteins. It has been implicated in various RNA-related processes particularly in mediating selective exon inclusion during alternative splicing and facilitating polyadenylation. This cell line can be used to study the role HnRNP-L plays in regulating chromatin modification as well as post transcriptional events and co-transcriptional pre-mRNA processing.
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemKidney
Cell MorphologyEpithelial
ApplicationsFor Research Use Only
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileFlag-tagged HnRNP-L
Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) MALDITOF/TOF; 2) RNase treatment and co-immunoprecipitation; 3) HKMT Assay; 4) RNAi knockdown of KMT3a and HnRNP-L; 5) Immunofluorescence

Supporting Protocol




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