Anti-GFP Tag Antibody (Mouse Monoclonal)
|Description||Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.|
|Unit quantity||1.0 mg|
|Specificity||Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP.|
|Immunogen||GFP from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates (first 20 aa).|
|Recommended Dilutions||WB 1:1000. For other applications, the researcher should determine the optimal working dilution.|
|Storage Condition||This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.|
|Storage Buffer||PBS, pH 7.4 with 0.05% sodium azide.|
|Guarantee||abm guarantees that all our Anti-GFP Tag Antibody (Mouse Monoclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.|
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