GFP Stably Expressing EOC 13.31 Cell Line
|T3965||1x106 cells / 1.0 ml|
|Description||EOC 13.31 cells stably expressing GFP|
|Species||Human (H. sapiens)|
|Applications||For Research Use Only|
|Unit quantity||1x106 cells / 1.0 ml|
|Propagation Requirements||The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003 to a final concentration of 69%. To make the completed growth medium, add the following components to the base medium: LADMAC conditioned media to a final concentration of 20%, fetal bovine serum (TM999) to a final concentration of 10%, 4.0 ug/ml puromycin (G264) and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.|
Carbon dioxide (CO2): 5%, Temperature: 37.0
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.
|Cell Type||Reporter Cells|
|Is the GFP or RFP signal localized within the cell?|
|What type of GFP or RFP do these cell lines express, and from which promoter?|
The GFP is derived from copGFP (from Pontellina plumata) and the RFP is derived from mKate2 (from Entacmaea quadricolor). They are expressed from a proximal SV40 promoter, and a more distal CMV promoter may also drive expression. If you are looking for a cell line with a specific fluorescent reporter, or with a specific promoter, abm can perform a Stable Cell Line Generation service. Simply ask [email protected] for a quote.
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