GFP Tag Lysate
| CAT.NO | UNIT | PRICE |
|---|---|---|
| L079 | 100 μg / 100 μl | $65.00 |
| Description | 293 cells transfected with GFP minigene, 30 kDa. |
|---|---|
| SKU | L079 |
| Lysate Type | Tag Lysate |
| Unit quantity | 100 μg / 100 μl |
Supporting Protocol
MSDS
QC
Other
| What is the buffer component for the cell lysates? | |
62.5 mM Tris-HCl
2% SDS
10% Glycerol
50mM DTT
0.01% w/v Bromophenol Blue | |
| I tried the Tag antibody on the corresponding lysate but do not get any signal on a western blot. Why? | |
Ensure the correct secondary antibody was used. Also, to get a good signal, try loading higher amounts of the lysate (such as 20ug) to strengthen the signal. | |
1
- Lloyd, T. E. et al. "Cytosolic 5′-Nucleotidase 1A As a Target of Circulating Autoantibodies in Autoimmune Diseases" Arthritis Care & Research 68(1): 66-71 (2015). DOI: 10.1002/acr.22600. Application: Cat. No. L032.