GFP Tag Lysate

CAT.NOUNITPRICE
L079100 μg / 100 μl
$65.00

Specifications


Description293 cells transfected with GFP minigene, 30 kDa.
SKUL079
Lysate TypeTag Lysate
Unit quantity100 μg / 100 μl
Documents


Supporting Protocol

    MSDS

      QC

        Other

          FAQs


          What is the buffer component for the cell lysates?
          62.5 mM Tris-HCl 2% SDS 10% Glycerol 50mM DTT 0.01% w/v Bromophenol Blue
          I tried the Tag antibody on the corresponding lysate but do not get any signal on a western blot. Why?
          Ensure the correct secondary antibody was used. Also, to get a good signal, try loading higher amounts of the lysate (such as 20ug) to strengthen the signal.
          References


          1
          • Lloyd, T. E. et al. "Cytosolic 5′-Nucleotidase 1A As a Target of Circulating Autoantibodies in Autoimmune Diseases" Arthritis Care & Research 68(1): 66-71 (2015). DOI: 10.1002/acr.22600. Application: Cat. No. L032.