GFP Tag Lysate
CAT.NO | UNIT | PRICE |
---|---|---|
L079 | 100 μg / 100 μl | $65.00 |
Description | 293 cells transfected with GFP minigene, 30 kDa. |
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SKU | L079 |
Lysate Type | Tag Lysate |
Unit quantity | 100 μg / 100 μl |
Supporting Protocol
MSDS
QC
Other
What is the buffer component for the cell lysates? | |
62.5 mM Tris-HCl
2% SDS
10% Glycerol
50mM DTT
0.01% w/v Bromophenol Blue |
I tried the Tag antibody on the corresponding lysate but do not get any signal on a western blot. Why? | |
Ensure the correct secondary antibody was used. Also, to get a good signal, try loading higher amounts of the lysate (such as 20ug) to strengthen the signal. |
1
- Lloyd, T. E. et al. "Cytosolic 5′-Nucleotidase 1A As a Target of Circulating Autoantibodies in Autoimmune Diseases" Arthritis Care & Research 68(1): 66-71 (2015). DOI: 10.1002/acr.22600. Application: Cat. No. L032.