Glutathione Agarose Resins
Affinity chromatography is a method of separating biochemical mixtures based on highly specific biological interactions. The Glutathione Agarose is developed for purification of fusion-proteins expressing a Glutathione S-transferase (GST) moiety. This GST-glutathione interaction makes one-step purification possible for proteins under native conditions. GST has a high affinity for its substrate, glutathione, which has been covalently bound to a solid support. Impurities are removed and the bound GST-fusion proteins can be eluted by using buffers containing reduced glutathione.
- Kim , BY et al. "Corneal Dystrophy-associated R124H Mutation Disrupts TGFBI Interaction with Periostin and Causes Mislocalization to the Lysosome" J Biol Chem 284 (29):19580-19591 (2009). DOI: 10.1074/jbc.M109.013607. Application: Protein Isolation Assays.
- How, KY et al. "Whole genome sequencing enables the characterization of BurI, a LuxI homologue of Burkholderia cepacia strain GG4" PeerJ : (2015). DOI: 10.7717/peerj.1117. Application: Protein Purification.