Glutathione Agarose Resins




Affinity chromatography is a method of separating biochemical mixtures based on highly specific biological interactions. The Glutathione Agarose is developed for purification of fusion-proteins expressing a Glutathione S-transferase (GST) moiety. This GST-glutathione interaction makes one-step purification possible for proteins under native conditions. GST has a high affinity for its substrate, glutathione, which has been covalently bound to a solid support. Impurities are removed and the bound GST-fusion proteins can be eluted by using buffers containing reduced glutathione.

Special Features
  • One-step easy purification protocol from crude lysate.
  • Purification under native conditions.
  • Can be used for proteins with low expression.
  • Can be used for successive cycles.
  • Ready-to-use for any scale of purification.
  • Protein binding capacity: approx. > 8mg recombinant GST/ml gel.
  • Resins and cartridges formats suitable for MPLC, FPLC, AKTA design, batch, gravity, peristaltic pump and syringe.
  • Beads are supplied as 75% (V/V) aqueous suspension (in 20% ethanol).
Resin TypeGlutathione
  • Purification of Glutathione-S-transferase (GST) and GST-tagged fusion proteins.
  • Antibody purification.
  • Assays involving protein-protein and protein-DNA interactions.
  • Structural and functional investigation of proteins.

Supporting Protocol





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          • Kim , BY et al. "Corneal Dystrophy-associated R124H Mutation Disrupts TGFBI Interaction with Periostin and Causes Mislocalization to the Lysosome" J Biol Chem 284 (29):19580-19591 (2009). DOI: 10.1074/jbc.M109.013607. Application: Protein Isolation Assays.
          • How, KY et al. "Whole genome sequencing enables the characterization of BurI, a LuxI homologue of Burkholderia cepacia strain GG4" PeerJ : (2015). DOI: 10.7717/peerj.1117. Application: Protein Purification.