HindIII

CAT.NOUNITPRICE
E07110000 U (500ul)
$90.00

Specifications


SKUE071
Unit quantity10000 U (500ul)
Applications

Restriction enzyme digestion of double-stranded DNA

Components

HindIII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)

Concentration20000 U/ml
Expression SystemAn E. coli strain that carries the cloned and modified HindIII gene from Haemophilus influenzae Rd (ATCC 51907). The HindIII enzyme recognizes and cuts the sequence A^AGCTT.
Inactivation Protocol80°C for 20 min
Reaction Definition1X Universal Restriction Enzyme Buffer (Cat.No. E204). Incubate at 37°C.
Reaction Buffer

Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%
Click here to view the buffer compatibility chart for restriction enzymes.

Storage Condition


-20°C

Storage Buffer0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 300 mM NaCl, 500 µg/ml BSA, pH 7.4 @ 25°C.
Note

Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Documents


Supporting Protocol
MSDS

    QC
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