HotStart DNA Polymerase (PCR)

G011250 U (50 µl)

Customize HotStart DNA Polymerase (PCR)
1 x HotStart Taq, small   + $0.00
1 x 10X PCR Buffer   + $0.00
1 x 25mM MgSO4   + $0.00

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HotStart DNA Polymerase (PCR)
HotStart DNA Polymerase (PCR)

In stock



    DescriptionProblems with non-specific amplification and primer-dimer formation? Try abm’s HotStart DNA Polymerase. HotStart DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, abm’s HotStart DNA Polymerase offers improved yield of desired PCR products. With abm’s HotStart DNA Polymerase, you can achieve • The highest specificity with minimal background • Superior performance • Improved yield of desired product
    Applications• Assays with prolonged reaction setup or liquid handling • Multiplex PCR • Specific amplification of difficult templates (i.e. G-C rich) • Low copy PCR assays • TA Cloning
    Concentration5 U/µl
    FormatEnzyme supplied with 10X buffer and MgSO4
    Note This product is ISO-13485 certified.
    Storage ConditionStore all components at -20°C.
    Unit quantity250 U (50 µl)

    Can HotStart Taq be used in multiplexing?
    Yes, however the end user may need to further optimize the enzyme amount per PCR reaction for multiplexing.

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