Integration-free wt1 iPSC
|T2512||1x106 cells / 1.0 ml|
|Description||Generated from human fibroblasts; Integration-free Wt1 iPSC is a clonal hiPSC line resulting from Sendai-virus based reprogramming. They have been validated to have a normal karyotype; lack any viral transgenes; show hESC-like expression profile; and display full differentiation competence into derivatives of the three germ layers. This cell line; originally created for use in human cardiac disease research; is suitable for disease modelling.|
|Species||Human (H. sapiens)|
|Applications||For Research Use Only|
|Seeding Density||20,000 cells/cm2|
|Cell Type||Ready-to-Use Stem Cells|
|Expression Profile||Normal karyotype; lack any viral transgenes; hESC-like expression profile|
|Preservation Protocol||40% hPSC culture medium; 50% Knockout Serum Replacement (Thermo); 10% DMSO; 10 uM Y-27632; 10 ng/ml FGF2 - or hPSC freezing media from commercial suppliers|
|Unit quantity||1x106 cells / 1.0 ml|
|QC||1) Karyotyping; 2) RT-PCR; 3) Illumina bead arrays for whole transcriptome analysis|
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