Integration-free wt1 iPSC

CAT.NOUNITPRICE
T25121x106 cells / 1.0 ml
$0.00

Specifications


DescriptionGenerated from human fibroblasts; Integration-free Wt1 iPSC is a clonal hiPSC line resulting from Sendai-virus based reprogramming. They have been validated to have a normal karyotype; lack any viral transgenes; show hESC-like expression profile; and display full differentiation competence into derivatives of the three germ layers. This cell line; originally created for use in human cardiac disease research; is suitable for disease modelling.
SKUT2512
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemSkin
Cell Morphologypolygonal
ApplicationsFor Research Use Only
Seeding Density20,000 cells/cm2
Donor GenderMale
Donor GenderMale
Donor AgeNewborn
Cell TypeReady-to-Use Stem Cells
Growth PropertiesAdherent
Expression ProfileNormal karyotype; lack any viral transgenes; hESC-like expression profile
Preservation Protocol40% hPSC culture medium; 50% Knockout Serum Replacement (Thermo); 10% DMSO; 10 uM Y-27632; 10 ng/ml FGF2 - or hPSC freezing media from commercial suppliers
Unit quantity1x106 cells / 1.0 ml
QC1) Karyotyping; 2) RT-PCR; 3) Illumina bead arrays for whole transcriptome analysis
Documents


Supporting Protocol

    MSDS

      QC

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