Klenow Fragment (3’→5’ Exo-)

CAT.NOUNITPRICE
E038500 U (100 μl)
$45.00

Customize Klenow Fragment (3’→5’ Exo-)
1 x Klenow Fragment   + $0.00
1 x 10X Klenow Rxn Buffer   + $0.00

* Required Fields

Your Customization
Klenow Fragment (3’→5’ Exo-)
Klenow Fragment (3’→5’ Exo-)

In stock

$45.00

Summary
    Specifications


    DescriptionKlenow Fragment (3’→5’ Exo-) is the large fragment of E. coli DNA Polymerase I which retains polymerase activity but lacks 5’→3’ exonuclease activity and has mutations that effectively abolish the inherent 3’→5’ exonuclease activity.
    SKUE038
    Unit quantity500 U (100 μl)
    Applications
    • Dideoxy DNA sequencing of single- or double-stranded DNA templates
    • cDNA second-strand synthesis
    • Generate single-stranded DNA probes using random primers
    • Site-directed DNA mutagenesis using synthetic oligonucleotides
    Concentration5 U/μl
    FormatEnzyme supplied with 10X Reaction Buffer
    Storage Condition

    Store all components at -20°C.

    Storage Buffer25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
    Note

    One unit is defined as the amount of Klenow Fragment (3’→5’ Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
    This product is distributed for laboratory research only. Caution: Not for diagnostic use.

    Documents


    Supporting Protocol
    MSDS

      QC

        Other

          FAQs


          There are no FAQs for this product yet!
          References


          There are no references for this product yet!